中国人群中HIV_1辅助受体CCR5基因新_省略_位点及CCR5_894C缺失基.pdf

1468 2002 11 10 82 21 Natl Med J China, November 10, 2002, Vol 82, No. 21 HIV1CCR5 CCR5894C 刘明旭 王福生 洪卫国 王波 金磊 雷周云 侯静 ! ∀ HIV1 CCR5 ( SNP) ;CCR5894C HIV1 CCR5 2PCR , 45 PCR ,DNAstar , SNP PCRRFLP ,HIV1,627CCR5894C 45 CCR5 6 SNP , 4 ( 184A# 503 # T 668 # A999 # T) ,; 1(894C ) , 184A# 503 # T999 # T SNP , 1. 11%, 21. 1% 10. 0%; 503 # T Hardy Weinberg CCR5894C 1. 11%0.53% 0 HIV1,HIV1 CCR5894C , CCR5 SNP ,4 SNP ( 3) , 1 CCR5894C , SNP HIV1 ! ∀ HIV1; ,CCR5; , ; Identification of new mutant sites and 894C deletion variant genotyping of HIV1 coreceptor CCR5 in indigenous Chinese populations LI U Mingxu , WANG F usheng ,HONG Weig uo, WANG Bo , JIN L ei, LEI Zhouyun , HOU Jing . PLA K ey L aboratory or Prevention and Therapy o Hep atitis AIDS , 302 Hosp ital o PLA , Beij ing 100039 , China ! A stract∀ O jective To investigate the new single nucleotide polymorphism ( SNP) sites of HIV1 coreceptor CCR5 gene and conduct genotyping of CCR5894C deletion allele in Chinese populations. Methods The full length fragment of CCR5 gene coding region was amplified by PCR amplification and sequenced in 45 healthy subjects from Han ethnic group. The sequencing data was analyzed by using DNAstar software for identification of new SNP sites. Furthermore, the genotyping of CCR5894C deletion variant was performed by PCRRFLP assay in 627 indigenous Chinese individuals including HIV1 carriers, HIV1high risk population of STDs or IDUs as well as normal contrast. Results Totally six SNPs and one cytosine base deletion at the 894 nucleotide of CCR5 gene coding region werefound in forty five Chinese Han subjects. Four SNPs, i.e.