t虫草素在体内外对小鼠睾丸间质细胞类固醇刺激作用.pdf

Biosci. Biotechnol. Biochem., 75 (4), 723–731, 2011 The in Vivo and in Vitro Stimulatory Effects of Cordycepin on Mouse Leydig Cell Steroidogenesis Sew-Fen LEU ,1; * Song Ling POON,2; * Hsiang-Yin PAO ,2 and Bu-Miin HUANG2;y 1Department of Food Science and Technology, College of Human Ecology, Hung Kuang University, Taichung, Taiwan, Republic of China 2Department of Cell Biology and Anatomy, College of Medicine, National Cheng Kung University, Tainan, Taiwan, Republic of China Received December 1, 2010; Accepted January 5, 2011; Online Publication, April 22, 2011 [doi:10.1271/bbb.100853] Cordycepin, a pure compound of Cordyceps sinensis receptors in the rat testis has been confirmed,20) but, the (CS), is known as an adenosine analog. We have found roles of adenosine in the testis remain unclear. Indeed, that CS stimulated Leydig cell steroidogenesis. Here we four different adenosine receptors that belong to the investigated the in vivo and in vitro effects of cordycepin family of guanylyl nucleotide-binding protein (G- in primary mouse Leydig cell steroidogenesis. The protein) G-protein coupled receptors (GPCRs) have results indicate that cordycepin increased the plasma been isolated in different tissues.21) These include the testosterone concentration. Cordycepin also stimulated A1 and A3 adenosine receptors (A1-AR and A3-AR), in vitro mouse Leydig cell testosterone production in which can interact with the inhibitory GTP-binding dose- and time-dependent manners. We further ob- protein (Gi),22,23) and the A2a and A2b adenosine served that cordycepin regulated the mRNA expression receptors (A2a-AR and A2b-AR), which interact with of the A1, A2