一测多评法同时测定桔梗中3 种桔梗皂苷的含量 - 中国现代应用药学.PDFVIP

一测多评法同时测定桔梗中 3 种桔梗皂苷的含量 1 2 蒋龄周 ，龚祖芳 (1.南通市第六人民医院，江苏 南通 226000 ；2.精华制药集团股份有限公司制剂研究所，江苏 南通 226000)  摘要：目的 探索一测多评法同时测定桔梗中 3 种桔梗皂苷含量的可行性。方法 采用 HPLC-ELSD 为检测手段，考察 桔梗提取物在不同色谱系统和不同色谱柱上的色谱行为，确定 3 种待测成分的色谱峰定位方法。以桔梗皂苷 D 为内标， 建立其与桔梗皂苷 D3 、桔梗皂苷 E 的相对校正因子；通过外标法测定桔梗皂苷 D 的含量，并由建立的相对校正因子计算 出其他2 种桔梗皂苷的含量；将计算的结果与外标法测定结果相比较，以验证“一测多评”法在桔梗质量评价中的可行性。 结果 方法重复性和耐用性良好，在 10 批桔梗药材的含量测定中，一测多评法计算结果与外标法测定结果无显著差异。 结论 一测多评法可用于同时测定桔梗中多种皂苷的含量。 关键词：桔梗；一测多评；桔梗皂苷 中图分类号：R284.1 ；R917.101 文献标志码：B 文章编号：1007-7693(2017)05-0729-04 DOI: 10.13748/ki.issn1007-7693.2017.05.022 引用本文：蒋龄周，龚祖芳. 一测多评法同时测定桔梗中 3 种桔梗皂苷的含量[J]. 中国现代应用药学, 2017, 34(5): 729-732. Determination of Three Ingredients in Platycodonis Radix by Quantitative Analysis of Multi-components by Single Marker 1 2 JIANG Lingzhou , GONG Zufang (1.The Sixth People’s Hospital of Nantong, Nantong 226000, China; 2.Preparation Research Institute, Jinghua Pharmaceutical Group Co., Ltd,, Nantong 226000, China) ABSTRACT: OBJECTIVE To study the feasibility of determination of three ingredients in Platycodonis Radix by quantitative analysis of multi-components by single marker(QAMS). METHODS With HPLC-ELSD as its detect method, chromatographic patterns of Platycodonis Radix extract in different chromatography instruments and columns were studied for positioning chromatography peaks of three ingredients. Then platycodin D was used as reference to create RCF with platycodin D3 and platycodin E, meanwhile, the content of platycodin D was determined by external standard method(ESM) and the contents of platycodin D and platycodin E were calculated by RCF. The results measured by ESM and QAMS were compared to 3 discuss the feasibility of determination of three ingr