cell apoptosis induced by lanthanum chloride - 第二军医大学学报.doc

氯化镧经caspase-3途径诱导子宫颈癌细胞凋亡 郭菲1* , 赵萍2 , 缪丽芳3 , 陆丹3 , 俞玲芳3 , 汪泱1* 1. 南昌大学第一附属医院烧伤研究所， 南昌，330006 2. 江西省科学院，南昌，330029 3. 南昌大学研究生院，南昌，330006 [基金项目] 国家自然科学基金 及江西省自然科学基金 (2007GZY1132). the National Natural Science Foundation of China , Natural Science Foundation of Jiangxi Province (2007GZY1132) [作者简介] 郭菲，博士，副研究员 *通讯作者（Corresponding authors）Tel 0791-8692539, 0791-8692527 Email:guofei2005@126.com,wangy63cn@ [摘要] 目 的 探讨氯化镧体外诱导人子宫颈癌细胞凋亡的作用和分子机制，为探索稀土化合物的药用价值提供理论依据。 方 法 采用形态学观察、噻唑蓝（MTT）比色法测定氯化镧对Hela细胞的抑制率；流式细胞术分析细胞周期和细胞凋亡率，免疫印迹技术分析Caspase-3蛋白表达的改变，并以分光光度法测定其活性。结 果 形态学分析显示氯化镧能够显著抑制Hela细胞生长并诱导其凋亡，其作用呈明显的量效关系。细胞周期实验结果说明，氯化镧可令细胞大多阻滞于G1期,并呈一定的浓度依赖性诱导细胞凋亡有剂量依赖性,在剂量下,细胞开始凋亡,随着药物浓度的增加,凋亡率不断增高, 浓度与对照组相比都具有统计学意义）Caspase-3蛋白表达。 GUO Fei 1*, ZHAO Ping 2, MIAO Li Fang3, Dan Lu3, YU Ling Fang3, WANG Yang 1* 1. Burns Institute, The First Affiliated Hospital of Nanchang University , Nanchang，Jiangxi province, China 2. Jiangxi Academy of Sciences，Nanchang，Jiangxi province, China 3. Postgraduate Department of Nanchang University, Nanchang, Jiangxi province, China [Abstract] Objective To explore the role of lanthanum chloride on inducing apoptosis and inhibiting cell growth in cervical cancer cells in vitro, which will provide a theoretical basis for medical use of rare earth compounds. Methods The anti-tumor activity of lanthanum chloride was detected by morphological observation and MTT (3-(4,5-Dimethylthiazo l-2-yl) -2,5- diphenyltetrazolium bromide) assay. Distribution of cell cycle and apoptosis were analyzed using flow cytometry (FCM). The expression and activation of Caspase-3 was measured by Western Blot and spectrophotometry. Results Morphological observation showed that lanthanum chloride can induce Hela cell apoptosis and inhibit the cell growth dose-dependently. FCM analysis showed that the cell ratio of G0/G1 phase increased and that of S phase decreased significantly with the increase of lanthanum chloride doses. Lanthanum treatment increased the