FBOX过表达影响子叶发育英文版.pptx

;content;1、the cotyledon and auxin ; 2、FBXL gene ; step① FBXL expression in Arabidopsis plants; GUS signals of FBXL in FBXL promotere:GUS transgenic Arabidopsis seedlings at 1e5 DAG. (a) 1, (b) 2, (c) 3, and (d) 5 DAG; Histochemical detection of GUS activity in 40 DAG FBXL promotereGUS transformed plants. (a) rosette leaf, (b) cauline leaf, (c) inflorescence, (d) root, and (e) magnified root image.; step② FBXL overexpression had no obvious effect on Arabidopsis seedlings; Phenotype of the cotyledons in 10 DAG FBXL-OE-1 or FBXL-OE-4 seedlings. WT, Wild-type; OE-1, FBXL-OE-1 plants; OE-4, FBXL-OE-4 plants.;step③： FBXL overexpression disrupted cotyledon vein patterning;result：① Abnormal cotyledon veins, such as incomplete or incorrect orientation veins,in wild-type plants occupied a low percentage (less than 1%). ②apparent abnormality was more than 40% of the total cotyledons observed in FBXL-OE-1 plants, whereas the ratio of plants with abnormal cotyledons was more than 60% in FBXL-OE-4plants. ;step④：FBXL gene expression was not regulated by exogenous auxin;Effect of 0.2 mg/l 2, 4-D on the distribution of FBXL transcript accumulation and auxin and hypocotyl elongation of FBXL-overexpressing transgenic seedlings. (a) 7 DAG DR5:GUS seedlings, (b) 7 DAG FBXL promotereGUS seedlings, (c) 7 DAG wild-type seedlings, and (d) 7 DAG FBXL-OE-4 seedlings.; Callus formation in FBXL-OE-4 seedlings grown on callus-inducing medium containing 1 mg/l 2, 4-D and 0.1 mg /l KT. (a) 20 DAG wild-type seedlings, (b) 20 DAG FBXL-OE-4 seedlings.;①FBXL was specifically expressed in the vascular tissues of the root, stem, leaf, andinflorescence. ②FBXL overexpression in Arabidopsis displayed an abnormal venation pattern in cotyledons. ③ FBXL expression was not induced by exogenous auxin and its transcript accumulation didnot overlap with the distribution of endogenous auxin. These results suggested that FBXL may beinvolved in cotyledon vein patterning via auxin-independent pathway.;THANKS