气相色谱法测定小麦胚芽油、大豆油和大蒜油中植物甾醇的组成和含量.PDF

7 8 Vol. 7 No. 8 2016 8 Journal of Food Safety and Quality Aug. , 2016 * 张喜金 , 魏鲜娥, 苏昭仑, 蔡伟江 (, 519040) 摘 要: 目的 方法 -, , 结果 , 0.99970.9999, 96.8%101.3%, 0.41.0%结论 β-, β-, 关键词: ; ; ; ; Determination of the composition and content of phytosterols in wheat germ oil, soybean oil and garlic oil by gas chromatography * ZHANG Xi-Jin , WEI Xian-E, SU Zhao-Lun, CAI Wei-Jiang (By-Health Co. , Ltd. , Zhuhai 519040, China) ABSTRACT: Objective To establish a method for the determination of the composition and content of phytosterols in wheat germ oil, soybean oil and garlic oil by gas chromatography. Methods Samples were saponified by potassium hydroxide ethanol-methyl tertiary butyl ether solution on water bath, and extracted with iso-octane. Phytosterols were separated by capillary gas chromatography. Results Phytosterols components showed a good linear relationship in the corresponding concentration range, correlation coefficients were from 0.9997 to 0.9999, the average recoveries were 96.8%~101.3%, and the relative standard deviations were between 0.4%~1.0%. Conclusion The phytosterols from wheat germ oil, soybean oil and garlic oil are mainly composed by campesterol, stigmasterol and β-sitosterol, and the highest content is β-sitosterol, the total amount of phytosterols in wheat germ oil is the highest. KEY WORDS: phytos