清脑复神液精制工艺再评价研究-天津中草药杂志社.PDF

中草药 =q==e=a 第 QS 卷 第 NS 期 =OMNR 年 U 月 ·OQMT · 清脑复神液精制工艺再评价研究 1 2 1 1 1 1 1* 刘 涛 ，伍利华 ，李鹏程 ，刘 婷 ，徐小艳 ，伍 月 ，徐玉玲 1. 成都大学，四川 成都 610106 2. 广西中医药大学，广西 南宁 530001 摘 要：目的 对清脑复神液精制工艺进行再评价研究，为改进该制剂的生产工艺提供实验依据。方法 以盐酸小檗碱转移 率、总黄酮转移率、固含物降低率为指标，采用正交试验优选活性炭吸附工艺；以盐酸小檗碱转移率、总黄酮转移率、固含 物降低率为指标，考察陶瓷膜孔径大小、滤过压力、滤过温度以及滤液收集量；同时采用不同的清洗剂组合筛选较优的膜清 洗方法。结果 活性炭吸附工艺为活性炭用量为 0.1%、温度为常温、吸附时间20 min ；滤过工艺条件为陶瓷膜孔径为200 nm 的微滤膜、压力0.10 MPa、温度40 ℃、滤液收集量为原体积的 1.25 倍；膜清洗方法为先采用 NaOH 循环冲洗 30 min，再 用 HNO3 循环冲洗 30 min 。结论 陶瓷膜分离技术用于精制清脑复神液效果较好，可产业化应用。 关键词：清脑复神液；预处理；膜分离技术；小檗碱；总黄酮 中图分类号：R284.2 文献标志码：A 文章编号：0253 - 2670(2015)16 - 2407 - 06 alfW 10.7501/j.issn.0253-2670.2015.16.011 oJ n=c l i 1 2 1 1 1 1 1 LIU Tao , WU Li-hua , LI Peng-cheng , LIU Ting , XU Xiao-yan , WU Yue , XU Yu-ling 1. Chengdu University, Chengdu 610106, China 2. Guangxi University of Chinese Medicine, Nanning 530001, China W l To reevaluate the purification process of Qingnao Fushen Oral Liquid (QFOL). j The orthogonal experiment of adsorption process of the activated carbon was optimized with the diversion rates of berberine and total flavonoids and the reduction of solid content as indexes. The aperture, pressure, temperature, and collection were optimized with the diversion rates of berberine and total flavonoids and the reduction of solid content as indexes. At the same time, the method of membrane cleaning was screened through the combination of different cleaners. o The adsorption process of activated carbon was as following: the usage of activated carbon was 0.1%, at normal atmospheric temperature, and the adsorption time was 20 min. The optimal process was aperture 200 nm, pressure 0.10 MPa, temperatu