南方根结线虫侵染黄瓜幼根组织病理学观察.pdf

2008, 13( 4) : 5 1-56 Journal of China A gricultur al Univ er ity 1 1, 2 2 1 刘奇志 李 娜 王 丽 谢德燕 ( 1. , 100193; 2. , 100 193) 人工接种南方根结线虫( M eloidogy n incog nita ) , 观察不同侵染时间黄瓜根部组织的病理特征结果显 示: 接种 6 d 的根尖分生区伸长区出现膨大对称的根结, 根中心的原形成层细胞分化出巨型细胞; 10 d 在根毛 区及以上部位出现既有对称又有偏于 一侧的根结, 周围有原生木质部导管出现, 根结中巨型细胞阻断了导管; 16 d 次生维管组织提早出现; 20~ 30 d 导管纵向排列无序, 形状扭曲, 皮层中有新维管组织产生总之, 随侵染时间的 延长, 根部受害部位逐渐由内皮层扩展至皮层, 根结逐渐由巨型细胞提早形成的次生维管组织以及皮层中的新维 管组织共同形成; 导管逐渐受阻, 排列扭曲无序 黄瓜; 南方根结线虫; 根结; 巨型细胞; 维管组织; 病理结构 S 436. 421. 29; S 432. 25 1007-4333( 2008) 04-0051-06 A Histopathological structure of cucumber radicle infected by root-knot nematode 1 1, 2 2 1 LI U Q-i zhi , LI Na , WANG Li , XIE De-yan ( 1. College of Agronomy and Biotechnology, China Agricultural Univer ity, Beijing 100193, China; 2. College of Biological Science, China Agricultural Univer ity, Beijing 100193, China) Abstract Patholog ical fe ature of a cucumber radicle ti ue inoculated with root-knot nematode ( RKN, Meloidogyn in- cog nita ) w ere ob erved on different day . Six day after inoculation, the cucumber ro ot tip wa lightly wollen and ymmetrical root-knot w ere ob erv ed at the meri tematic and elong atio n zone . Giant cell were differentiate d by pre- v iou c ambium cell in the c enter of the root; 10day after inoculation, ymmetrical and eco ndary ro ot-knot w ere ob- erved at the ro ot hair and upper zo ne . Ve el were eparated by g iant cell , around which protoxylem ve el were