抗鹅免疫球蛋白轻链单克隆抗体的鉴定及其应用详解.PDF

生 物 工 程 学 报 郭永丽 等/抗鹅免疫球蛋白轻链单克隆抗体的鉴定及其应用 1531 Chinese Journal of Biotechnology /cjbcn November 25, 2016, 32(11): 1531−1538 DOI: 10.13345/j.cjb.160149 ©2016 Chin J Biotech, All rights reserved 动物及兽医生物技术 郭永丽，高明春，罗修鑫，鞠环宇，安东，刘莹，王君伟 150030 , , , . . , 2016, 32(11): 1531−1538. Guo YL, Gao MC, Luo XX, et al. Characterization and application of a monoclonal antibody against light chain of goose immunoglobulin. Chin J Biotech, 2016, 32(11): 1531−1538. 摘 要: 免疫球蛋白是机体固有免疫系统的组成部分，是机体防御的第一道防线。本研究对抗鹅免疫球蛋白 轻链单克隆抗体进行了特征分析并将其应用到不同免疫试验中用以检测鹅免疫球蛋白。用此单克隆抗体制备的 免疫亲和层析柱用以分离血清中的鹅免疫球蛋白；偶联辣根过氧化物酶 (Horseradish peroxidase ，HRP) 后的单 克隆抗体用作第二抗体来检测鹅特异性抗体。此外，该单克隆抗体可以识别和定位外周血淋巴细胞中的 SIg+淋巴 细胞。研究表明，该单克隆抗体可在多种条件下检测或分离鹅免疫球蛋白并作为研究鹅体液免疫的有力工具。 : 单克隆抗体，鹅免疫球蛋白，轻链，免疫试验 Characterization and application of a monoclonal antibody against light chain of goose immunoglobulin Yongli Guo, Mingchun Gao, Xiuxin Luo, Huanyu Ju, Dong An, Ying Liu, and Junwei Wang College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, Heilongjiang, China Abstract: Immunoglobulin (Ig) is considered a part of the innate immune system and cooperates with the complementary system as the first line of defense. In this study, a monoclonal antibody (MAb) direct against the light chain of goose Ig (GoIgCL) was generated, characterized and identified in various immunoassays to detect goose Ig. An immunoaffinity chromatography column prepared with this MAb was used to separate the goose Ig from sera. After being conjugated with horseradish peroxidase (HRP), this MAb was used as the secondary