- 1、本文档共7页,可阅读全部内容。
- 2、有哪些信誉好的足球投注网站(book118)网站文档一经付费(服务费),不意味着购买了该文档的版权,仅供个人/单位学习、研究之用,不得用于商业用途,未经授权,严禁复制、发行、汇编、翻译或者网络传播等,侵权必究。
- 3、本站所有内容均由合作方或网友上传,本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺!文档内容仅供研究参考,付费前请自行鉴别。如您付费,意味着您自己接受本站规则且自行承担风险,本站不退款、不进行额外附加服务;查看《如何避免下载的几个坑》。如果您已付费下载过本站文档,您可以点击 这里二次下载。
- 4、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等,请点击“版权申诉”(推荐),也可以打举报电话:400-050-0827(电话支持时间:9:00-18:30)。
查看更多
考马斯亮蓝显色剂变色反应机理的研究
2002 60 Vol. 60, 2002
9 , 1656~ 1661 ACTA CHIMICA SINICA No. 9, 1656~ 1661
a, b X , a a a
曹稳根 焦庆才 刘 茜 陈 雷
a
( 210093)
b
( 234000)
G-250 ( CBBG) , CBBG
. , CBBG y y ,
CBBG .
, , ,
Study on the Mechanism of Color Changes of Commassie
Brilliant Blue G-250
a, b X , a a a
CAO, Wen-Gen IAO, Qing-Cai LIU, Qian CHEN, Lei
a
( Pharmaceutic Biotechnology Key Laboratory, College of Lfie Science , Nanj ing University, Nanj ing 2 0093)
b
( Dep artment of Biochemistry, Suzhou Teachers. College, Suzhou 234000)
Abstract The mechanism of color changes of Commassie Brilliant Blue G-250 ( CBBG) has been investigated
by spectrometry. The influence of different experimental conditions on the absorption spectra of CBBG was
inspected. It is found that CBBG forms aggregate by hydrophobic interaction in high phosphoric acid
concentration. Due to the difference in the aggregative degree, CBBG shows color changes from blue to green to
brown in the system.
Keywords Commassie Brilliant Blue G-250, mechanism of color changes, spectrometry, hydrophobic
interaction
, .
, ,
[ 8, 9]
. , ,
. 197
文档评论(0)