固相萃取-气相色谱-质谱联用法测定葱姜蒜中12种农药残留量.pdfVIP

萄街农业学报 2016 年29 卷9 期 2138 Southwest China Joumal of Agricultura1 Scienc臼 Vol.29 No.9 文章编号:1∞1 -4829(2016)09 -2138-06 DOI: 10. 16213/j. cnki. scjas. 2016. 09. 022 固相萃取-气相色谱-质谱联用法 测定葱姜蒜中 12 种农药残留量 梅文泉，陈兴连，汪禄祥，邵金良，耿慧春，王 丽 (云南省农业科学院质量标准与检测技术研究所农业部农产品质量监督检验测试中心，云南昆明 650223 ) 摘 要:建立了固相萃取-气相色谱-质谱联用法同时测定葱姜蒜中 12 种农药残留量的方法。试样经乙精提取后，采用氟罗里 硅土固相萃取小柱净化，然后用气相色谱-质谱联用法对 12 种农药进行定量分析。结果表明，采用氟罗里硅土固相萃取小柱净 化，能有效去除葱姜蒜样品中的色素、油脂等杂质，净化后得到的样液清亮、透明。 12 种农药在 0 ∞5 - O. 22 mglkg 添加水平下的 回收率为 81. 0%-120% ，相对标准偏差为 2.33 %句 16.2 % ， 12 种农药检出限为 O. ∞3 - 0.05 mglkg。 关键词:葱;姜:蒜;农药;残留;测定 中图分类号:0657.63 文献标识码:A Determination of 12 Pesticide Residues in Onion , Ginger and Garlic by GC/MS-SPE MEI Wen-quan , CHEN Xing-lian , WANG Lu-xiang , SHAO Jin-liang , GENG Hui-chun , WANG Li (Quality Standardizin!( and Testing Technology Institute , Yunnan Academy of A伊cultur址 Science ， Supervision Testing Center for Farm Products Quality , Minist巧 。f Agricultu宣誓， Yunnan Kunming 650223 , China) A恼tract: The method for dP!ermination of 12 pesticide ,,;idues in onion , ginger and garlic w植 developed. The samples were extracted by acetonitrile , purifiecl with florisil solid phase extraction , and quantified the 12 pesticides by GC!MS. The [它sults showed 也at it could remove 由e impurities of pigment , lipid in sample using florisil 50lid phase extraction. The sample solution after purified was clear and 国nslucent. Under these conditions , the average recoveries of 12 pesticides ranged from 81. 0 % to 120 % at spiked level ofO.05 -0.22 mgl峙， and the rel