固相萃取-高效液相色谱法测定中药制剂及保健食品中芍药苷.pdfVIP

(PART B: CHEM. ANAL.) 固相萃取-高效液相色谱法测定中药制剂 及保健食品中苟药苦 高广慧1 ，田金苗2 ，赵飞1 ，孙晓娟l (1.辽宁省食品检验检测院，沈阳 110015; 2. 辽宁省药品检验检测院，沈阳 110023) 摘要:应用固相萃取-高效液相色谱法测定中药制剂及保健食品中巧药苦的含量。样品经 色谱柱为分离柱，用乙睛和 30%(体积分数)乙醇溶液提取，酸性氧化铝固相萃取柱净化。以 C 18 0.1%(体积分数)磷酸溶液以不同比例混合的溶液为流动相进行梯度洗脱，在检测波长 230 nm 处 进行测定。巧药苦的质量浓度在 2. 00~200 mg • L一1 范围内与其峰面积主线性关系，方法的检出 F~(3S/N) 为 0.5 mg. L一1 。加标回收率在 99. 5%~101%之间，测定值的相对标准偏差(η=6)在 均小于 2%。 关键词:高效液相色谱法;固相萃取;中药制剂;保健食品;苟药苦 中圄分类号: 0652.63 文献标志码:A 文章编号: 1001-4020(2015)02-0228-03 HPLC Determination of Peoniflorin in Traditional Chinese Medicine and Health Food with Solid Phase Extraction GAO G uang-huP , TIAN Jin-miao2 , ZHAO Fei1 , SUN Xia o-juan 1 (1. Liaoning Provincial Institute for F，∞d Co饲trol. Shenyang 110015. China; 2. Liaoning Provincial Institute for Drug co咽trol. Shenyang 110023. China) Abstract: HPLC was applied to the determination of peoniflorin in traditional Chinese medicine and health food with solid phase extraction. The sarnple was extracted with 30 % (ψ) ethanol solution. and purified by passing through acidic Abαsolid phase extraction colurnn. C18 chromatographic column was us.ed as stationary phase with mixtures of acetonitrile and O. 1% (ψ) H3 P04 solution with different mixing ratios as mobile phase in gradient elution. UV-detection at 230 mh was adopted in the dete口runation. Linear relationship between values of peak area and mass concentration of peoniflorin was kept in the range of 2. 00-200 mg. L一1. with detection limit (3S/N) of 0.5 mg. L一1 Values of