土壤及植物复合体系中有机酸的测定.pdfVIP

第 33 卷第9 期 Vol.33 No.9 饨，电亏品.ti#. 2010 年 9 月 Environmental Science Technology Sept. 2010 土壤及植物复合体系中有机酸的测定 孙宝利， 赤杰， 范中南， 贺小蔚， 李艳华， 全乘风* (中国农业科学院农业环境与可持续发展研究所; 农业部农业环境与气候变化重点实验室，北京 10∞81) 摘 要:通过对 10 种有机酸的色谱条件试验、样品处理试验(以 4C和一20C作为样品运输和储存条件)以及加标回收率试验，建立 并优化了土壤及植物复合体系中 10 种有机酸的测定方法。方法添加回收率在 74:协9210 ， 变异系数CV;;;4.1%，方法的最低检测限为0.2阳 50昭Ikg，应用该方法测定了番茄植物及其土壤样品中的有机酸，结果表明该方法能够满足测量田间环演条件下士壤及植物复合体系中 mglkg 痕量级有机酸含量要求。 关键词:土壤及植物复合体系; 有机酸; 测定 中图分类号:X830.2 文献标志码:A doi: 10.3969/j.issn.1003-65ω.2010.09.027 文章编号:1ω3-6504(2010)09-0130-05 Determination of Organic Acids from Integrated System of Soil and Plant SUNBa。一li， CHI Jie , FAN Zhong-nan , HE Xiao-wei , LI Yan-hua , TONG Chen-feng* (Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences; .Key Laboratory of AgroeJlvironmen~ and Climate Change of MÍlùstry of Agriculture , Beijing 100081, China) Abstract: A method of determination of trace 1巳vel of 10 kinds of organic acids from integrated system of soil and plant was established through experimentation of HPLC instrument, sample prep缸ation under s缸nple transportation and conver- sation at 4-20C respectively and recovery trails at different fortification levels, with recoveries , coefficient variations and lirnit of detection of the method as 74%-92%，运4.1%， 0.2-50μglkg respectively. Results of the .determination of tomato plants and its soil showed that the method could accurately monitor 也巳 trace level of 10 kinds of organic acids 扭曲e int巳­ grated system of soil and plant. Key words: integrated system of soil and plant; organic acid; determination 低分子量有机酸广泛存在于植物体内、植