在线凝胶色谱-气质联用测定卷烟主流烟气中的苯并[a]芘.pdfVIP

烟草科技I Tobacco Science Technology 烟草化学I Tobacco Chemistry 2014 年第 9 期(总第 326 期) 在线凝胶色谱-气质联用测定卷烟主流烟气中的苯并[a] 花 段玩杏，王昆森，刘志华，张 霞，杨光字，韩融美，赵 伟，缪明明 云南中烟工业有限责任公司技术中心，昆明市五华区红锦路367 号 650032 摘要:建立了准确测定卷烟主流烟气中苯并[a] 花 (B[a]P) 的在线凝胶色谱-气质联用(在线 GPC-GC/MS) 分 析方法。卷烟主流烟气依次经剑桥滤片捕集、环己炕萃取后进行在线GPC-GC/MS 测定，以苯并[a] ;t -dI2 (B[a]P-dI2 )为内标进行定量。结采表明:B[a]P 在 0.4-50 ng/mL 浓度范围内线性关系良好(R=0.9995) ， 3 个 加标水平的回收率在95.6%-97.29毛之间，平均相对标准偏差(RSD)2.18% ， 检出限0.28 ng/ 支。该方法灵敏 度高、定性定量结果可靠，适用于卷烟主流烟气中 B[a]P 的测定。 关键词:在线 GPC-GC/MS; 苯并[a] 花;卷烟;主流烟气 中图分类号:TS41 1. 2 文献标识码:A 文章编号: 1002-0861(2014)09-0039-05 Determination of Benzo[a]pyrene in Mainstream Cigarette Smoke by Online Gel Permeation Chromatography-Gas Chromatography/Mass Spectrometry DUAN Yuanxi吨， WANG Kunmiao , LIU Zhihua, ZHANG Xia , YANG Guangyu , HAN Jingmei , ZHAO Wei , and MIAO Mingming Technology Center, China Tobacco Yunnan lndustrial Co. , Ltd. , Kunming 650032 , China Abstract: ln order to accurately determine the benzo[a]pyrene (B[a]P) in mainstream cigarette smoke , an online gel permeation chromatography-gas chromatography/mass spectrometry (online GPC-GC/MS) method was developed. The B[a]P in mainstream cigarette smoke was collected by a Cambridge filter pad , then the pad was extracted with cyclohexane , the extract was quantitatively analyzed by online GPC-GC/MS with B[a]P-d12 as an intemal standard. The results showed that: B[a]P displayed good linearity (R=0.9995) in the range of 0 .4 to 50 ng/m L. The recoveries at three spiked levels ranged from 95.6% to 97.2% with the mean relative standard deviation of 2.18% and the limit of detection of 0.28 ng/cig. This method is reliable , sensitive and accurate in qualitative and quantitative analyses , and suitable for the determination of B[a]P in mainstream cigarette smoke. Keywords: Online gel permeation chromatography-gas chromatography/mass s