基于邻氟苯基荧光酮荧光探针的荧光光谱法测定牛血清白蛋白.pdfVIP

PTCA(PARTB: CHEM. ANAL.) 001: 10. 11973/1hjy-hx201603004 基于邻氟苯基荧光回荧光探针的荧光光谱法 测定牛血清白蛋白 王艳妮，马红燕祷，高春燕，权银 (延安大学化学与化工学院陕西省反应工程重点实验室，延安 716000) 摘要:在 pH 7.40 的磷酸盐缓冲溶液中，牛血清白蛋白(BSA) 可使邻氟苯基荧光嗣(o-FPF) 荧光猝灭，卢环糊精的加入可使荧光猝灭强度( t:.F) 增强，据此建立了一种基于 o-FPF 为荧光探针 测定 BSA 的荧光光谱法。在选定的试验条件下，BSA 浓度在 6. OX lO-9~4. OXIO一7 mol • L一1 范 围内与 t:.F 呈线性关系，方法检出限(3s/均为 7. 1 X 10-lomol • L一l 。该方法用于测定牛奶、牛奶粉 中的蛋白质含量，结果与考马斯亮蓝法测定结果相吻合，测定值的相对标准偏差(n=5) 小于 4%。 通过测定荧光寿命、探讨温度对猝灭常数的影响以及紫外吸收光谱的变化，确定BSA 与 o-FPF 之 间的猝灭过程为静态猝灭。 关键词:荧光光谱法;牛血清白蛋白;邻氟苯基荧光嗣;作用机理 中图分类号: 0657.3 文献标志码:A 文章编号: 1001-4020(2016)03-0266-05 Determination of Bovine Serum Albumin by Fluorescence Spectrometry with o-Fluorophenylfluorone as a Fluorescent Probe WANG Yan-ni , MA Hong-yan瞥， GAO Chun-yan , QUAN Yin CShaan:ri Key Laboratory of Chemical Reaction Engineering , College of Chemistry and Chemical Engineering. Yanan University , Yanan 716000. China) Abstract: In PBS with pH 7.40 , bovine ser田n albumin CBSA) could quench the fluorescence intensity of 0-- fluorophenylfluorone (o--FPF) and the fluorescence quenching intensity (t.F) was enhanced by the addition offrC D. Based on this. a method of fluorescence spectrometry with o--FPF as a fluorescent probe was proposed for the determination of BSA Under the optimized conditions , a linear relationship was found between t.F and the 9 7 concentration of BSA in the range of 6. 0 X 10- - 4. 0 X 10- mol • L寸. with the detection limit C3s/k) of 7. 1 X lO-)Omol. L -). The method was applied to the determination of BSA in milk and