复方柴术片中芍药苷和甘草酸的HPLC-DAD法测定.pdfVIP

中间医药工业杂志 Chinese Joumal of Pharmaceuticals 2009. 40 (2) 103 • -~~~，~，，，，~总1 5 中葫与天然药物 3 盯~.民c:c;c，c.- 复方柴术片中均药营和甘草酸的HPLC-DAD法测定 郭春11，沈卫阳1* (1.中闰药科大学分析化学教研室，药物质量去与安全预警教育部主意点实验攘，南京 210009， 2.南京市药品检验所，南京210015) 摘要3 建立了HPLC法同时测定复方柴术片中苟药督和甘毒草酸的含量。采用C 柱，流动相为乙膀-0.01%磷酸水溶液， I8 梯股洗脱，检测波长240 nm. 句药f牙利甘草酸的线性描围为6.18-370.8μg/ml(r= 0.9999) 和5 .47-328.2μg/ml(r- 0.9999) ，平均回收率为96.1 %(RSD 1.0%)和95.0% (RSD 0.9%) 。 关键询:复方柴术片:苟药管:甘尊酸:高效液相缸谱p 测定 中朋分提号:民917 文献标识码:A 文章编号: 1001-8255 (2009) 02-0103-03 Determination of Paeoniflorin and Glycyrrhizic Acid in Fufang Chaishu Tablets byHPLC皿DAD 1 1 GUO Chun ,2 , SHEN Wei嗣yang * (1. Dept. ofAnalytical Chemis的\ China Pharmaceutica/ University, Nanjing 210009; 2. Nanjing 1nstituteforDrug Con仰1， Na.η;ing 210015) ABSTRACT: An HPLC method was established for determination of paeoniflorin and glycyrrhizic acid in f句fang Chaishu tablets. A C18 column was used with the mobile phase of acetonitrile-O.Ol % H P04 by gradient elution. 3 ηle detection wavelength was 24仇lDl. The calibration curves of paeoniflorin and glycyrrhizic acid were Hnear ín the concentration range of 6.18 蝇 370.8μglml (阳0.9999) and 5.47 蟠 328.2μglml(严0.9999) .ηle average recoveries of paeoniflorin and glycyrrhizic acid were 96.1 % (RSD 1.0%)and 95.0% (RSD 0.9%) ,respectively. Key Words: Chai协u tablets; paeoniflorin; glycyrrhizic acid; HPLC; determination 复方柴术片是由柴胡、白苟、甘草等七昧药制 草酸(glycyrrhizic acid , 2) 含壤。 成的复方制制，以四逆散为药材方