大叶藜叶中总黄酮含量的测定.pdfVIP

安徽农业科学，Journal of Anhui Agri. Sci.2015 ,43(34) :164 -165 责任编辑李占东责任校对况玲玲 大叶黎叶中总黄嗣含量的测定 武芸1 ，马世荣2 ，王春林2 ，胡浩斌1 ，张小伟1 (1.陇东学院化学化工学院，甘肃庆阳 745000 卢陇东学院生命科学与技术学院，甘肃庆阳 745000) 摘要 [目的]建立大叶黎叶中总黄嗣含量的测定方法。[方法]采用分光光度法，以芦丁为对照品，10 %KOH 为显色剂，在403 nm 处对 样品中的总黄嗣含量进行测定。[结果]总黄嗣在 10.7-64.2μg/ml 范围内呈良好的线性关系， R =0.997: 平均加样回收率为98.450毛， RSD 为 0.560串 (n =6) ，大叶黎叶中的总黄嗣含量为 2.45 mg/go [结论]该方法稳定、简便、快速，适用于大叶黎叶中总黄嗣含量的测定。 关键词 紫外-可见分光光度法;大叶苯;总黄嗣;含量测定 中图分类号 55日.21 +9:R284.1 文献标识码 A 文章编号 0517 -6611(2015)34 -164 -02 Detennination of Total F1avone in the Leaf of Chenopodium hybridum L. wu Yun , MA Shi-ron矿， WANG Chun_lin2 et al ( 1. College of Chemistry and Chemical Engineering , Longdong University , Qingyang , Gansu 745仪lO :2. College of life 5cience and Technology ,Longdong University , Qingyang ,Gansu , 745αlO) Abstract [Objective] To establish a method for the total flavonoid content determination of the leaf of Chenopodium hybridum 1. [Method] ηle content of total flavones from the leaf of Chenopodium hybridum 1. was determined by UV -visble spectrophotometry with liquiritin as refer- ence substance ,10% KOH as chromogenic agent at the detection wavelength of 403 nm. [Result]ηle total flavonoid showed a good linear re- 2 lationship at 出e range of 10.7 - 64. 2μ在Iml ， R =0.997. Tbe average recoveηrate was 98.45% ,RSD = 0.560毛 Total flavone content in the leaf of Chenopodium hybridum was 2. 45 mg/g. [Conclusion] Tbe method was ralatively simple ,convenient and accurate ,and could be used for tbe quality control for Chenopodium hybridum 1. Key words UV -visble spectrophotometry: Chenopodium hybridum: Total flavonoids: Content determination 大叶毒草为毒草科( Chenopodiaceae )事属 ( Chenopodium ) 一 1. 3 方法 年生草本植物，别名杂配馨、八角灰菜、血见愁等，我国各地 1. 3.1 供试品榕液的制备。准确称取干燥至恒重的原料 均有分布[l] ，其地上部分可作中药，主治月经不调、子宫出