大豆提取液中大豆黄素的紫外分光光度测定法.pdfVIP

盒品研究与持发 2013 年2 月 幢测分析 Food Research And D们elopment 第 34 卷第4 期 77-==- DOI: l0.3969/j.issn.lOO5-6521.2013.04.023 大豆提取液中大豆黄素的 紫外分光光度测定法 蒋盼，顾建明 (上海大学生命科学学院，上海2ω钊4) 摘 要:紫外分光光度法(248 nm)测定大豆提取液中的大豆黄素浓度与 HPLC 法溃1] 得的值有显著差异(t 检验法)。以 HPLC 法测得的值为可信佳，用函数的方法对紫外法标准曲线进行矫正，矫正后的紫外法标准曲线方程为 y=ü.706 lx+ 0β125 ，回收率为 99.128 %，精密度为 5.387 %，用此方程测得的样液中大豆黄素浓度与 HPLC 法测得的值元显著差 异(t 检验法)。 关键词:大豆异黄朗;大豆黄素;测定;紫外分光光度法;高效液相色谱法 UV Spectrometry Method to Determine Daidzein in Soybean Extract JIANG Pan ,GU ]ian-ming (School of Life Sciences ,Shanghai University ,Shanghai 200444 ,China) Abstract: There was significant difference between the two daidzein values in the soybean extract determined by UV (at 248 nm) and HPLC (t test ,tω1) UV standard curve was corrected by mathematic method and HPLC values as truth-values ,a new relationship between concentration (y )and absorbency (x) ,y=O.706 lx+O.OI2 5 , was established ,with no significant difference between UV and HPLC ,the recoveηof99.128 % and the relative standard deviation (RSD )of 5 .387 % for daidzein detection. Key words: soybean isoflavone; daidzein; determine; ultraviolet spectrometry; HPLC 异黄酣(isoflavone)在自然界中的分布只局限于豆 相似，能维持女性雌激素水平的平衡，缓解妇女更年 1 期综合症I问;(2)预防骨质酥松症(8-9 ; (3)调节血脂以 科的蝶形花亚科等少数植物中，其中含量较高的有茵 1