大鼠经皮给药血浆中多奈哌齐浓度的UPLC-MSMS法测定.pdfVIP

• 中国医药工业杂志 Chinese Joumal of Pharmaceuticals 2012 , 43 (1 0) • 849 • ~，，，，，，，，，~~ i 药品分析与质控 2 ~(c..c:.c:.c..cc.‘~， ..ζ‘民 u...忌 大鼠经皮给药血浆中多奈服齐浓度的 UPLC-MS/MS 法测定 式余泼，朱壮志，罗华菲，陈纯，王浩* (中国医药工业研究总院药物制剂国家工程研究巾心，上海 201203) 摘要z 建立了超高效液相色谱-串联质谱法测定大鼠血浆中的多条赈齐。以氯雷他定为内标，液-液萃取处理血浆样品， 采用 ESI 源正离子模式、多反JiY.U任测进行定量分析。监测离子对为 mlz 380.3 • mlz 243.2 (多奈赈齐)和 m/z383.1→m/z 337.1 (氯雷他定)。多奈赈齐在 0.5 ~ 200 ng/ml 范围内线性关系良好，方法回收率为 94.l%~ 106.8% ，日内、日间 RSD 均小于 10% 。 关键词:多奈贩齐:超高效液相色谱-串联质i普:测定 中图分类号: 0657T2: R969 文献标志码:A 文章编号: 1001-8255 (2012) 10-0849-04 Determination of Donepezil in Rat Plasma after Transdermal Administration by UPLC-MS/MS WU Yubo , ZHU Zhuangzhi , LUO Huafei , CHEN Chun , WANG Hao* (National Pharmace川cal Engineeri咆Research Center, China State Institute 01Pharmaceutical Indust.叨 Shanghai 201203) ABSTRACT: An UPLC-MS/MS method was established for the determination of donepezil in rat plasma with loratadine as the intemal standard. The plasma samples were pretreated by liquid-liquid extraction. The mass spectrometer was used in the multiple reaction monitoring (MRM) with the ESI-positive mode. The MRM was perfo口ned at m/z 380.3• m/z 243.2 for donepezil and m/z 383.1 • m/z 337 .l for loratadine. The calibration cu凹e of donepezil was linear in the concentration range of 0.5 - 200 ng/m l. The method recoveries were between 94.1 % - 106.8%. The intra- and inter- day RSDs were less than 10%. Key Words: donepezil; UPLC-MS/MS; determination , 多奈赈齐 (donepezil， 1) 为特异的可逆性乙酷 目前已有很多文献报道了用 HPLC 法测定生物样品 中的1，较常用的有紫外 (uv) [2] 、荧光 (FL) [3] 和 胆碱醋酶 (AchE) 抑制剂， I 临床用于改善轻中度阿