小柴胡颗粒中柴胡皂苷a和柴胡皂苷d的毛细管电泳法测定.pdfVIP

中国医药工业杂志 Chinese Journal of Phannaceuticals 2012 , 43 (1) 47 • 小柴胡颗粒中柴胡皂昔a 和柴胡皂昔d 的毛细管电泳法测去E 易润青，宋粉云* (广东药学院，广东广州 510006) 摘要z 建立了毛细管电泳法测定小柴胡颗粒中的柴胡皂营a 和柴胡皂背d。以苯甲酸为内标，采用未涂层弹性融在圭翠玉萝茎 毛细管柱，以20 mmol/L Tris+20 mmol/L 磺丁基-ß- 环糊精+25 mmoUL ß- 环糊精 (pH 9.93) 为运行缓冲液，分高重吨主主正 12 kV. 重力进样 10 s(高度 15 cm). 检测波长210 nm。柴胡皂背a 和柴胡皂背d 在 10 - 100 和 8-80μg/ml 浓居主奇主自章 内线性关系良好。平均回收率为 97.1%和 98.1%. RSD为1.57%和1.98% 。 关键词z 小柴胡颗粒:柴胡皂营a; 柴胡皂昔d; 毛细管电泳法:测定 中图分类号: TQ460.7+2; 0657.8 文献标志码:A 文章编号: 1001-8255 (2012) 01-0047-04 Determination of Saikosaponin a and Saikosaponin d in Xiαochaihu Granules by Capillary Electrophoresis YI Runqing , SONG Fenyun* (Guangdong Pharmaceutica/ University, Guangzhou 510006) ABSTRACT: A capillary electrophoresis method was estab 1ished for the determination of saikosapo:n.量I1 a anl saikosaponin d in Xiaochaihu granules. An uncoated fused si 1ica capi11ary column was used with benzoic a.c孟d as 由 intemal standard and 20 mmo 1lL Tris + 20 mmo l/L SBE-ß-CD + 25 mmo l/L ß-CD bu证er solution (pH 9_93) as th running buffer at the voltage of 12 kV. The sample was injected by gravity (1 0 s , 15 cm). The detection wavel~gth wa 210 nm. Their calibration curves were linear in 也e concen甘ation ranges of 10 - 100μgIrnl and 8 - 80μglml.The量.r a.verag recoveries were 97.1 % and 98.1 %, with RSDs of 1.57% and 1.98% . Key Words: Xiaochaihu granules; saikosaponin a; saikosaponin d; capillary electrophoresis; determination 小柴胡颗粒是《伤寒论》中经典方小柴胡汤的 胡主要的有效成分之一为柴胡皂昔，其中妻是由月皂t 现代制剂，由柴胡、黄苓、姜半夏、党参、生姜、 a (1)和柴胡皂昔 d (2) 活性较强。本研究重主五立了三毛 甘草和大枣七味药材制成，具有解表散热、疏肝和