川贝清肺糖浆中苦杏仁苷、甘草苷和甘草酸的双波长UPLC法测定.pdfVIP

中国医药工业杂志 Chinese Joumal of Pharmaceuticals 2015 , 46 (8) • 889 • 川贝清肺糖浆中苦杏仁昔、甘草音和甘草酸的双波长 UPLC 法测定 陈建琴 (南宁食品药品检验所，广西南宁 530001) 摘要:建立了超高效液相色谱法同时测定川贝清肺糖浆中的苦杏仁青、甘草苦和甘草酸。采用 Agilent Eclipse Plus C8 色谱柱，以乙睛 0.1 %磷酸为流动相，梯度洗脱，双波长切换检测 (0~7min， 210nm 测定苦杏仁苦、甘草音; 8 ~ 14 min , 250 nm 测定甘草酸)。苦杏仁营、甘草苦和甘草酸分别在 0.9 ~ 8.3 , 0.2 ~ 1. 7 和 0.4 ~ 3.4陆Iml 浓度范围内线 性关系良好。平均回收率分别为 100.7% 、 100.8%和 99.5% ， RSD 分别为0.42%、 0.56%和 0.71% 。 关键词: )11 贝清肺糖浆1 苦杏仁背:甘草苦:甘草酸:超高效液相色谱:测定 中图分类号: 0657.7+2; TQ460.γ2 文献标志码:A 文章编号: 1001-8255 (2015) 08-0889-03 DOI: 10. 16522/ki.cjph.2015.08.02 1 Determination ofAmygdalin , Liquiritin and Glycyrrhizic Acid of Chuanbei Qingfei Syrup by UPLC with Double-wavelength CHEN Jianqin (Nanning Institutefor Food and Drug Control, Nanning 530001) ABSTRACT: A UPLC method was established for the determination of amygdalin , liquiritin and glycyηhizic acid of Chuanbei Qingfei syrup. An Agilent Eclipse Plus Cl8 column was used , with the mobile phase of acetonitrile : 0.1 % phosphoric acid by gradient elution and double-wavelength switching detection. The detection wavelength was set at 210 nm during 0 - 7 min for the determination of amygdalin and liquiritin , and then changed to 250 nm for the determination of glycyrrhizic acid during 8 - 14 min. It was linear for amygdalin , liquiritin and glycyηhizic acid in the ranges of 0.9 - 8.3 , 0.2 - 1.7 and 0.4 - 3.4 μglml， respectively. Their average recoveries were 100.7%, 100.8% and 99.5% , with RSDs of 0.42 %, 0.56 % and 0.71 %, respectively. Key Words: Chuanbei Qingfei syrup; amygdalin; liquiritin; glycyrrhizic; UPLC; determination 川贝清肺糖浆主要由批把叶、苦杏仁、甘草等