应用酶重量法测定全麦粉的总膳食纤维.pdfVIP

回 dff企TMM 川，No.8 doi:10. 7633/j. issn.1003-6202. 2015. 08. 017 应用酶重量法测定全麦粉的总膳食纤维 张春华1 ，徐广超2 ，苏宁辰1 ，陈吉江l (1.中粮营养健康研究院有限公司，北京 100020; 2. 国家粮食局标准质量中心，北京 100037) 摘 要:膳食纤维具有重要的营养作用和保健功能，酶重量方法是测定食品中膳食纤维比较方便和经济的一种方 法。参考国标 GB/T 22224-2008 ，建立了一种测定全麦粉膳食纤维含量的方法。采用磷酸缓冲液代替 MES-TRIS缓冲液，比较了磷酸缓冲液和 MES-TRIS 缓冲液对检测结果的影响。结果表明，使用 MES-TRI 缓冲 液和磷酸缓冲液对测定结果影响不大，精密度均较好，方差分别为 0.10 和 0.01 。两种方法测定结采的绝对差值 与平均值之比最大为 4.62% ，低于GB/T 22224-2008 中允许的双试验误差 10% 。 关键词:全麦粉;总膳食纤维;酶重量法;测定 中固分类号:TS210. 7; TS207. 5 1 ;0657.3 文献标志码:A 文章编号: 1003-6202 (2015) 08- 0069 一02 Determination of total dietary fiber of whole wheat f1 0ur by enzyme gravimetric method 1 2 1 1 Zhang Chunhua ,Xu Guangcha0 ,Su Yuchen ,Chen Jijiang (1. COFCO Nutrition and Health Research Institute Co. , Ltd. , Beijing 100020 , China; 2. Quality Standard Center , State Administration of Grain , Beijing 100037 ,China) ABSTRACT: Dietary fiber has an important role in nutrition and health care function. The method of enzyme gravimetric is a convenient and economical method for the determination of dietary fiber in food. According to GB/T 22224-2008 ,a method for determination of total dietary fiber content of wheat flour was established. The effect of phosphate buffer and ME8-TRIS buffer on the detection results was compared. The results showed that the effects of ME8-TRI buffer solution and phosphate buffer on the determination results were not significant , the precision were both good ,and the variance was O. 10 and 0.01 ,respectively. The maximum ratio of absolute deviation and average one by the two methods was 4. 62 % ,which was lower than the double test error 10 % in GB/T 22224• -2008. KEYWORDS: whole wheat flour; total dietary fiber; enzymatic gravi