微乳液相色谱法对蓝莓叶黄酮类化合物的分离和测定.pdfVIP

※成分分析 国国融事 2015, Vo1.36, No.14 91 微乳液相色谱法对蓝莓叶黄嗣类化合物的 分离和测定 赵丹1 ，苏宁2 ，郑拱艳2 ，杨丽2 ，霍彤1 ，王昌涛M (1.北京工商大学理学院，北京 1【削48; 2. 中国检验检疫科学研究院，北京 1∞123) 摘 要z 目的:利用微乳液相色谱分离并测定蓝莓叶中儿茶素、芦丁、山奈酣、懈皮素和杨梅酣5 种黄酣成分。 方法:从表面活性剂、助表面活性剂、 pH值、有机相4 个方面对微乳液相色谱分离蓝莓叶黄国的条件进行优化。 结果:分离蓝莓叶黄国的最优条件为:使用2.5%聚氧乙烯 (23) 月桂酶作为表面活性剂， 8%正丁醇为助表面活 性剂， 0.8% 乙酸乙酶为有机相， 0.5% 乙酸调节体系pH值为3.2 ，在以上条件下蓝莓叶黄嗣在20min内能够得到有 效分离。结论z 蓝莓叶中提取的黄圄类化合物含有儿茶素9.76 mglg、芦了13.12 mglg、山奈酣3 1.04 mglg、懈皮素 26.42 mglg、杨梅嗣20.30 mglgo 关键词:蓝莓叶:黄酣类化合物:微乳液相色谱:分离:测定 Isolation and Quantification of Flavonoids in Blueberry Leaves by Microemulsion Liquid Chromatography 2 2 2 1 ZHAO Dan\ SU Ning , zl狂到G Hongyan , YANG Li , HUO Tong , WANG Changtao1.* (1. Sch∞1 of Science, Be动ing Technology and Business University , Beijing 1αlO48， China; 2. Chinese Academy of Inspection and Quarantine, Beijing 1∞123， China) Abstract: Purpose: To optimize the procedure for isolating flavonoids from blueberry leaves by microemulsion liquid chromatography (MELC) and to quantify 由e flavonoids obtained under the optimum conditions. Methods: Four MELC conditions including surfactant, cosurfactant, pH of the mobile phase and organic phase were optimized. Results: The optimum chromatographic conditions were determined as follows: 2.5% B均-35 act as the surfactant, 8% l-butanol as the cosurfactant, the mobile phase adjusted to pH 3.2 with 0.5% acetic acid, and 0.8% ethyl acetate as the organic solvent. l The tlavonoids in blueberry