沙棘果油的酶法提取及其脂肪酸的测定.pdfVIP

2602011，VoL32，No．02 良晶科学 ※分析检测 沙棘果油的酶法提取及其脂肪酸的测定 康健，顾晶晶，王继国，吴桐，张丽霞 (新疆大学生命科学与技术学院，新疆乌鲁木齐830046) 摘 要：目的：对酶法提取沙棘果油及其脂肪酸测定进行相关研究。方法：通过正交试验确定酶泫提取沙棘果油 的最佳T艺条件。结果：果胶酶用量O．50％、胃蛋白酶用量0．15％、提取时间6h、酶解温度55℃，此条件下果 7．26％，酶法提取沙棘果油中不饱和脂肪酸的含量占总脂肪酸的61．56％。结论：酶法提取沙棘果油安全温和而无 毒害，反应彻底，提取率高，提取后的果汁易于进行澄清处理。 关键词：沙棘；果油；酶法提取；测定 ExtractionofSeabuckthomFruitOilandIts AcidsProfile Enzymatic Fatty Analysis KANGJian，GU Li-xia Jing-jing，WANGJi·guo，WUTong，ZHANG ofLifeScienceand (College Technology，XinjiangUniversity，i．Jriirnqi830046，China) Abstract：Fortheextractionofseabucthomfruit and wergusedto seabucthom oil．pectinasepepsin simultaneouslyhydrolyze fruit the extractionwas factor incombinationwiⅡl enzymatic process by pulp．and optimizedsingle experiments orthogonal werefound amaximumoil of4．946％wasachievedunderthese the ofthe condidons。and optimum，and yield recovery tO extractionwascalculatedbe82．16％一97．17％basedon5．09％一6．02％theoreticaloncontentof optimized process seabucthornfruit．In acid ofseabucthornfruitoilwas GC-MS．Eleven addition，the acids fatty composition analyzedby fatty weleidentifiedinseabucthornfruit acid,12．77％linoleic oil，mainlyincluding35．cr7％palmitoleicacid，35．6l％palmitic acid, 6．19％cis一1l-octadecenoicacid,and unsaturatedacids 61．56