液相色谱-串联质谱测定小麦粉中11种真菌毒素.pdfVIP

-==l56 液相色谱一串联质谱测定小麦粉中 11种真菌毒素 李磊1，李海畅2，周贻兵1。林野1 (1．贵州省疾病预防控制中心，贵州贵阳550004；2．贵阳市疾病预防控制中心，贵州贵阳550002) 摘要：建立小麦粉中11种真茵毒素的超高效液相色谱一串联质谱检测方法。样品用乙腈一水溶液提取，经多功能净化 柱PriboFast 效液相色谱一串联质谱进行分析。11种真菌毒素在测定浓度范围内线性关系良好，各组分相关系数R20．998，加标回收 率范围72．4％-90．7％，方法精密度范围6．2％11．8％，测定各组分真茵毒素定量限范围1．2 la,g／kg一2．0t,eCkg。该方法前处 理过程简洁、灵敏度高、重现性好，适用于小麦粉中11种真茵毒素的测定。 关键词：小麦粉；真茵毒素；超高效液相色谱一串联质谱 in Performance Determinationof11 WheatFlour Mycotoxins by Liquid Mass Spectrometry LI Yel Leil，LI Hai-chan92，ZHOUYi-bin91，LIN ofCenterforDisease and (1．GuizhouProvincialandChemical Control Physical Inspection Prevention，Guiyang Disease ProvincialCenterfor Controland 550002， 550004，Guizhou，China；2．Guiyang Prevention，Guiyang Guizhou，China) Establishmethodtodetectthe11 kindsof inwheatflourultra Abstract：Objects mycotoxins by performance mass werecrushedandsievedextracted liquidchromatography—tandemspectrometry．MethodsSamples by column XterraMSC18 muhifunctional PriboFast aqueousacetonitrile，purifiedby cleaning 1130，separatedby mmol／Lammonium detected eluted20 acetateand acetonitrile，and