- 1、本文档共65页,可阅读全部内容。
- 2、有哪些信誉好的足球投注网站(book118)网站文档一经付费(服务费),不意味着购买了该文档的版权,仅供个人/单位学习、研究之用,不得用于商业用途,未经授权,严禁复制、发行、汇编、翻译或者网络传播等,侵权必究。
- 3、本站所有内容均由合作方或网友上传,本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺!文档内容仅供研究参考,付费前请自行鉴别。如您付费,意味着您自己接受本站规则且自行承担风险,本站不退款、不进行额外附加服务;查看《如何避免下载的几个坑》。如果您已付费下载过本站文档,您可以点击 这里二次下载。
- 4、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等,请点击“版权申诉”(推荐),也可以打举报电话:400-050-0827(电话支持时间:9:00-18:30)。
查看更多
基因工程2-2
11-17-2004 Molecular Biology Genetic Engineering Introduction++ Mutation and Mutagenesis变异发生++++ Gene Expression++++ Bacteriophage++ Plasmids, Transposon and Vectors+++ rDNA Cloning ++++ PCR Technology ++++ Medical Forensic Application++ Mutation and Repair DNA faces a constant challenge. Damages during DNA replication, if not corrected, could lead to permanent永久的change-mutation Most mutations are potentially潜在地dangerous危险的, so DNA repair system needed for correcting replication errors and for eliminating消除damage to DNA caused by environmental agents and intracellular chemicals Chapter 2: Mutation and Mutagenesis Term and definition Types of mutations+ Mutagenesis Mutagen Reversion复原 DNA Repair Mechanisms Mutagenesis in vitro体外+ I.Term and definition1 Mutant: an organism or a gene that is different from the normal or wild type, or an organism in which either the base sequence of the DNA or the phenotype has been changed + as wild type, and - as a mutant Mutation: any heritable可遗传的change in the base sequence of a DNA molecule. The most common type of change is a substitution置换, addition, or deletion删除of one or more bases I.Term and definition2 Spontaneous mutation: mutation occurs in nature without the addition of a mutagen and the resulting mutant is spontaneous mutant. It may arise through (a) errors in DNA replication or (b) spontaneous alteration of a nucleotide within an existing DNA molecule Induced mutation: the process is caused by a mutagen Mutagenesis: the process过程of producing a mutation I.Term and definition3 Mutagen: a physical or chemical agent that causes mutations to occur (or increases the frequency of their occurrence) Hot spot: a site is represented by as many as 100 times the typical number of mutation Mutator gene增变基因: a gene that, when presents in a mutant state, causes mutations to appear very frequently in other genes throughout the genome II. Types of Mutations Mutation by Base Number Change Base substitution Frameshift移码Mutation Muta
文档评论(0)