基于酶反应的微生物检测.ppt

Easy and fast colorimetric system to carry out microbiological analyses on FOOD AND WATER “Micro Biological Survey?” - MBS? ProductMBS HACCPWATER Easy test Multireader Reaction vials TechnologyMBS HACCPWATER Easy test The MBS method measures the catalytic activity of the redox enzymes in the main metabolic pathways of bacteria through a redox indicator that changes color according to the oxidative state of the medium. The time required for the color change is inversely related to the logarithm of bacterial concentration and this allows to obtain an unequivocal correlation between the observed enzymatic activity and the number of viable cells present in the samples. NADH NAD+ I III II Q c 2 H2O 4H+ + O2 H+ H+ H+ IV TMPD NAD+ I III II Q c 2 H2O 4H+ + O2 H+ H+ H+ IV TMPD OUT OUT TechnologyMBS HACCPWATER Easy test In the presence of bacteria the indicator will cause colour change in the reaction vials. The greater the number of living bacteria, the faster the colour change. Selectivity is assured by the peculiar composition of each reagent and from the temperature of analysis: only target bacteria are able to grow in the reaction vials. 0 100 0 10 Change of colour Time for colour change (hours) The MBS method has been developed in order to meet the need to provide reliable results, thus diminishing the time of analysis, facilitating procedures and interpretation of data, increasing the detection sensitivity, limiting costs and allowing analysis also in the absence of a laboratory. It usually does not require sample pre-treatment and media preparation. The main features are: Ease of use Speed Accuracy Ready to use, portable and disposable devices Automation Analysis of both solid and liquid sample No sample pretreatments required Sterilization post-analysis included FeaturesMBS HACCPWATER Easy test Analysis availableMBS HACCPWATER Easy test MBS reagents that have been developed so far for the analysis of food and water samples. The already available