内毒素在PAAF诱导的肠损伤中的作用研究.pdfVIP

1854 Guangxi Medica2 Joaftra2，Nov．2007，Dec．29，No．12 内毒素在PAKF诱导的肠损伤中的作用研究 方 向 程若川 刘 震 陈玉祥 李 俊 倪邦高 (1四川省德阳市人民医院普外科，德阳市 618000：2昆明医学院普外科，昆明市 650032) 【摘要】 目的 探讨内毒素在胰腺炎相关性腹水(PAAF)诱导的大鼠肠损伤中的作用及其相关机制。方法 以PAAF诱导 的sD大鼠肠损伤模型为研究对象，将32只sD大鼠(雌雄不拘)随机分为2组，每组16只。对照组腹腔内注射生理盐水8 ml／只； 实验组腹腔内注射PAAF 8 ml／只。于6、12 h分批处死大鼠，每时间点8只。测定血清内毒素和血白细胞(WBC)计数，大鼠呼吸频 率计数，测定小肠组织A rP酶的活性。结果 同时问点比较：实验组血清内毒素、WBC呼吸频率均高于对照组(P0．05)，A rP酶 活性显著降低(P0．01)。结论 内毒素在PAAF诱导的大鼠肠损伤中具有重要的作用。 【关键词】 胰腺炎；腹水；肠黏膜损伤；内毒素 【中图分类号】 R 657．51 【文献标识码】 A 【文章编号】 0253-4304(2007)12—1854-02 Effect of the endotoxin on the intestinal mucosal injury in rats induced by PAAF FANG Xing，CHENG Ruo—chuan，LIU Zhen，CHEN Yu—xiang，LI Jun，LI Bang-gao (1 Department ofGeneral Surgery，Hospital ofDeyang City，Sichuan Province 618000 China； 2 Department of General Surgery，First Affiliated Hospital ofKunming Medical CoZlege，Kunming 650032，China) 【Abstract】 Objective To investigate the effect of endotoxin on intestinal mucosal injury in rats induced by PAAF．Methods Twen~healthy Sprague—Dawwley(SD)rats，10 male，were made into acute necrosis pancrosis pancreatitis models．Thirty—two healthy SD rats，16 male，were randomly divided into two groups：group A served as the control and group B as the model group，the rats were put to death in two baches at 6 hours and 12 hours，eight rats per batch．LeVels of endotoxln serum and WBC in blood and the breathing rate，were measured．The activity of A11P enzyme in the intestinal tissues were measured．ResIllts The levels of endotoxin in serum and WBC in blood．the breathing rate，the level of endotoxin in the intestinal tissues were markedly increased and the activity of ATP enzyme in the intestinal tissues was decreaded in the group B compared with those in group A(P0．05)．Conclusion Endotoxin plays