消毒剂中污染菌对消毒剂抗性及耐药性研究.pdfVIP

中国卫生检验杂志2007年l2月第l7卷第l2期 Chinese Journal of Health La[x~ratory Technology，Dec 2007；Vol 17 No 12 2261 【监测报告】 消毒剂中污染菌对消毒剂抗性及耐药性研究 何明 ，曾斤日 ，许业栋 ，丁华 ，陈伟光 ，刘镇平 (1．广东省深圳市宝安区沙井人民医院，广东深圳 518104；2．广东省深圳市宝安区沙井卫生监督所，广东深期f 518104) [摘要] 目的：了解社区医疗消毒剂中污染菌抗消毒剂性和耐药性情况。方法：对来自使用中消毒剂的9株芽胞杆菌进 行细菌定量杀灭试验，同期对45株污染菌进行药敏试验。结果：在以ATCC9372为参考菌的最低杀菌浓度和杀菌时间 下，对9株污染菌进行细菌定量杀灭试验的杀菌效果均未达到消毒学要求；45株试验污染菌对CRO等8种抗生素敏感 性显著下降，且出现二重、多重，甚至六重耐药。结论：试验污染菌对消毒剂抗性增强，试验污染菌耐药谱中CRO—M—P 耐药谱型比例最大。 [关键词] 使用中消毒剂；污染菌；细菌定量杀灭试验；耐药谱；抗性 [中图分类号] R187 [文献标识码] B [文章编号] 1004—8685(2007)12—2261—03 Study of resistance of contaminated bacteria isolated from in——used disinfectant to disin-- fectants and drug He Yue ，Zeng Jin．ri ，Xu Ye．dong ，肪 Hua’，Chen Wei·guang’，Liu Zhen-ping (1．Shajing Provincial People S Hospital of Baoan，Shenzhen 518104，China；2．Shajing Health Inspection of Baoan，Shenzhen 518104，China) [Abstract] 0bjeetive：To understand resistance of contaminated bacteria isolated from in—used disinfectant to disinfectants and drug．Methods：The quantitative bactericidal test was used on nine strains of bacilli from in—used disinfectants．Meanwhile， the drug susceptibility test was used on forty～five strains of contam inated bacteria．Results：The sterilization effects of quantitative bactericidal test to above contaminated bacteria are not required to antisepsis within a bactericidal concentration taking ATCC9372 as reference bacteria．The sensitivity of eight types antibiotics including CRO decreased significantly，and appeared double—re· sistance，multi—resistance，even six—resistance,Conclusion：The resistance of contam inated bacteria to disinfectant is increas． ing．The proportition of CRO—M—P—spectrum resistance in resistance spectrum is the largest． [Key words] In—used dis