深圳市某校一起诺瓦克病毒性胃肠炎疫情调查分析.pdfVIP

中国卫生检验杂志2007年12月第l7卷第l2期 Chinese Journal of Health Laboratory Technology．Dec 2007：Vol 17 No 12 【调查报告】 深圳市某校一起诺瓦克病毒性胃肠炎疫情调查分析 袁梦，陈辉，刘楚云，温群文，许艳子，李维克，俞慕华 (广东省深圳市南山区疾病预防控制中心，广东深圳 518054) [摘要] 目的：调查以发热呕吐、腹泻为主要症状的胃肠炎的病因，查找传染源，以采取相应的控制措施。方法：采用流 行病学调查方法对病例进行调查，对病人以及食堂员工肛拭、粪便以及环境样品采用实时荧光PCR进行定性检测。结 果：经诺瓦克病毒基因检测，学生样本3例阳性；教师食堂员工样本阴性；环境样本1例阳性。6月15、19日对73名学生 食堂员工进行诺瓦克病毒基因检测，分别有7例(9．6％)和5例(6．9％)阳性。25日，再次对73名食堂员工进行检测，全 为阴性。通过测序分析比对，从食堂员工以及学生中检测出的诺瓦克病毒为同一型诺瓦克病毒。结论：该校学生胃肠炎 是由诺瓦克病毒感染引起，学生食堂员工带毒是引起此次诺瓦克病毒发生流行的传染源。 [关键词] 诺瓦克病毒；实时荧光PCR；胃肠炎；序列分析 [中图分类号] R373．2 5 [文献标识码] B [文章编号] 1004—8685(2007)12—2298—02 Epidemiological investigation and analysis of an outbreak of norwalk virus gastroenteritis at a school in Shenzhen Yuan Meng，Chen Hui．Liu Chu—yun，Wen Qun—wen，Xu Yan—zi，血Wei·ke，Yu Mu·hurt (Nanshan Center for Disease Control and Prevention，Shenzhen 518054，China) [Abstract] Objective：To investigate the source and transmission of gastroenteritis at a school in Shenzhen，Methods：Cases were analyzed by the epidemiological method，and feces of patients and eatery staff were detected by the Real—Time Fluorescent PCR．Results：Samples of the students，teachers and eatery staff and environment were tested by Real—Time Fluorescent PCR of Norwalk virus．Samples from three students and one environment were positive and sam ples of the teacher S eatery staff were nega— tive．Samples 0f 73 teacher s eatery staft were examined by Real—Time Fluorescent PCR of Norwalk virus gene on June 15出and 19 ．Respectively the 7(9，6％)and 5(6．9％)samples were positive．Samples from the same 73 teacher S eatery staff were col— lected and detected again on June 25 m and all negative．The result of sequence analysis showed the same type of Norwalk virus in positive sam ples．Conclusion：The outbreak of gastroentefitis in t