溃疡性结肠炎大鼠一氧化氮和氧自由基的变化及其相关性研究.pdfVIP

国基层医药2007年11月第l4卷嵬 l 朔 —bin J Prim Med Ph—arm,November 2007,Vo1．14,No．11 · 1763 · 溃疡性结肠炎大鼠一氧化氮和氧自由基的变化 及其相关性研究 刘建生 田 怡 张晓红 刘海玲 孙 菁 袁耀宗 吴云林 【摘要】 目的 研究大鼠溃疡性结肠炎(UC)血、肠黏膜中一氧化氮(NO)和超氧化歧化酶(SOD)变化及 丹参对其影响。方法 予雌性Sprague—DawIey大鼠含30 mg三硝基苯磺酸(TNBS)的50％乙醇溶液0．85 ml 灌肠，诱导实验性溃疡性结肠炎。造模7 d后，健康对照组、模型组尾静脉注入0．9％NaC1溶液 1 ml／d；丹参 治疗组每天尾静脉注入丹参注射液2 m1．kg ·d-。。通过肠道重量指数、溃疡面积、大体形态和组织学评分 及血和肠黏膜中NO、SOD含量测定评估疗效。结果 丹参治疗组肠道重量指数、溃疡面积、大体形态和组织 学评分及血和肠黏膜中NO含量减少、SOD含量增高，与模型组比较差异有统计学意义(P0．01)；但较健康 对照组NO含量仍增高和SOD含量减少(P0 05)。结论 UC大鼠血、肠黏膜中NO和SOD异常．可能是 UC发生和发展主要因素之一；丹参有效治疗UC与其拮抗NO、氧自由基机制有关。 【关键词】 结肠炎，溃疡性；一氧化氮；超氧化歧化酶；丹参 Stady of the nitric oxide，oxygen free radical in serun and intestine mucosal in rats LIU Jian—sheng ，Tian Yi． ZHANG Xia0一hong，L，U Hai ling，SUN Jing．YUAn4 Yao—zong、w【厂run—lin． Depart*nenl of Digestiwe Dis- eases，The Ruoin Hospital Bloc Minkang Hospital，Shahghai 20 1 1 00，China 【Abstract】 Objective To explore the changes of nitric oxide(NO)and superoxide dismutase(SOD)in the serun and intestine nlucosaj and the treatment Of ulcerative colitis(UC)with tanshinon in rats．Methods 30 mg of trinitro-benzene sulfonic acid(TNBS)dissolved in 0．85ml of 50％ ethanol was administrated intrarectally in Sprague—Dawley female rats tO induce experimental colitis、Mter 7 days，the rats were dMded into normal control， 0、9％ saline and treatment group tanshinon 2ml‘kg一 ‘d一 were intravenously．The therapeutic effect was evaluated by measuring the ponderal index，the surface area of the ulcers、macroscopical and histological SCOre、activity of NO and SOD were measured in colonic tissue and serum all rats．Results C~mpared with the saline group，the ponderal index，the surface area of the ulcers，macroscopical and histological score，activity of NO level in the serunl and intes- line mucosal was decreased and the SOD increased of significantly in the treatment group(P0．01)、while activity of NO level was increased and tile SO D was decreased significantly in the tanshinon grou