滇重楼的花粉活力测定方法比较.pdfVIP

安徽农业科学，Journal ofAnhui Agri．Sci．2007，35(35)：11451，11453 责任编辑 张杨林 责任校对 王淼 滇重楼的花粉活力测定方法比较 王定康1，荆、桂芳2，郭志明3，王荔芳4岑晓江 (1．昆明学院生物系，云南昆明650031；2．昆明医学院教务处，云南昆明650032； 3．云南省宣威市农业局，云南宣威 655400；4．云南大学信息学院，云南昆明 650091) 摘要 f目的1为提高滇重楼的繁殖效率。f方法1将滇重楼开花植株裂开花药的花粉轻轻涂抹载玻片上，分别用碘一碘化钾(I：．KI)染色 法、氯化三苯四氮唑(TTc)染色法、醋酸洋红染色法和略有改动的荧光素二醋酸酯法对滇重楼花粉活力进行测定。f结果】氯化三苯基四 氮唑染色法的染色率较低，仅为27．51％；醋酸洋红染色法和荧光素二醋酸酯法的染色率较高，分别为91．30％和90．55％，而且所测 数据较为接近；碘一碘化钾染色法虽然能将花粉染上色，染色率为94．80％，但染上色的花粉包含退化的花粉和失活的花粉。 }论1醋 酸洋红染色法和荧光素二醋酸酯染色法的染色率较高，能较好地区分出有活力的花粉和失活的花粉，而且能较为准确地反映滇重楼 花粉的生活力。 关键词 滇重楼；花粉活力；荧光素二醋酸酯；染色率 中图分类号 Q944．58 文献标识码 A 文章编号 0517—6611(2o07)35—11451—01 Comparison of Determination nethods of Paris polyphylla var．yunnanensis Pollen Activity WANG Ding-kang et al(Department of Biology，Kunming College，Kunming，Yunnan 650031) Abstract f0biective1 The study aimed to increase the reproduction efficiency of Par／s polyphyUa var．yunnanensis．fMethod1 The pollens of dehisced anthors of flowering P．polyphflla plants was slightly painted on the slide glass．the activities of P．polyphylla pollens were determined resp．by i0dine-p0tassium iodide dyeing method(I2-KI)，triphenyl tetrazolium chloride staining method(1rrc)，aceto carmine dyeing method and fluorescein diacetate method with a Iittle improvement．fResult1 The dyeing rate of T】 method was Iower，being only 27．51％；that of aceto carmine dyeing method and fluorescein diacetate method were higher，being 91．30％ and 90．55％resp．，and the determined data were close to each other．Although I，一KI method could dye pollens with dyeing rate of 94．80％．the dyed pollens included degraded and inactive ones． fConclusion1 Aceto carmine dyeing method and fluorescein diacetate method has higher dyeing rate．The active and inactive pollens can be distinguished better and the viability of P pol~phylla pollens can be reflected more accurately． Key words Par／s polyphylla var．yunnanensis；Pollen activity；Fluorescein diacetate；Dyeing rat