珍珠层水溶性基质对兔骨髓基质干细胞BMP-2和Cbfα1基因表达的影响.pdfVIP

· 1838· 南方医科大学学报(J South Med Univ) 珍珠层水溶性基质对兔骨髓基质干细胞BMP一2和CbfoL1基因表达的影响 王建钧，陈建庭，杨春露(南方医科大学南方医院脊柱骨病科，广东 广州 510515) 摘要：目的从珍珠层水溶性基质(WSM)对兔骨髓基质干细胞分泌的骨形成蛋白．2和核心结合因子基因表达的影响人 手，研究WSM产生成骨作用的机制和途径。方法培养新西兰大白兔的骨髓基质干细胞(BMSCs)．并用低温下提取的 珍珠层水溶性基质作用后，检测不同浓度WSM作用下碱性磷酸酶的活性，制定剂量．效应曲线，确定量效关系：采用 一 步RT—PCR方法检测不同浓度WSM诱导兔BMSCs后骨形成蛋白．2和核心结合因子的基因表达量并进行统计学 分析。结果WSM可以增加兔BMSCs中碱性磷酸酶的活性．在 l50～200)~g／ml浓度之间作用明显．碱性磷酸酶活性增 加显著；WSM诱导兔BMSCs后骨形成蛋白的表达量明显增加，核心结合因子的表达量无明显变化。结论WSM通过增 加骨形成蛋白．2的基因表达量诱导兔BMSCs向成骨细胞分化．与核心结合因子的作用关系不明显。 关键词：珍珠层水溶性基质；骨髓基质干细胞；骨形成蛋白I2；核心结合因子 中图分类号：R3l8．08 文献标识码：A 文章编号：1673-4254(2007)l2．1838．03 Effects of soluble matrix of nacre on bone morphogenetic protein-2 and Cbfal gene expressions in rabbit marrow mesenchymal stem cells WANG Jian-jun，CHEN Jian-ting，YANG Chun—lu Department ofOrthopedic and Spine Surgery，Nanfang Hospital，Southern Medical University,Guangzhou 510515，China Abstract： Objective To provide insights into the mechanisms and pathways of osteogensis by observing the effects of water-soluble matrix of nacre (WSM)on bone morphogenetic protein一2 (BMP一2)and Cbfal gene expressions in rabbit malTOW mesenchymal stem cells MSCs1． Methods New Zealand rabbit BMSCs cultured in vitro were stimulated m different concentrations Of WSM extracted at low temperature． and the activity Of AKP in the cells was evaluated with the dose．effect calve generated． BMP一2 and Cbfa1 gene expressions in rabbit BMSCs exposed tO WSM were assayed wim one．step IU’．PCR．Results The activity OfAKP in rabbit BMSCs increased after stimulation with different concentrations of WSM． and the effects were the most obvious wjm the WSM concentration ranging from 150 tO 200)~g／m1． BMP一2 gene expression in the BMSCs increased after WSM exposure． but which did not result in obvious changes in Cbfal gene expression． Conclusion WSM induces differentiation of rabbit BMSCs towards ost