益舒软肝丸抗脂质过氧化的实验研究.pdfVIP

实用医学进修杂志 2007年第35卷第4期 JOURNAL OF PRACTICAL 1 N G 0F mDICINE V 35№ 4 Dec．捌『7 · 209· 益舒软肝丸抗脂质过氧化的实验研究 陈 超 王希君 三峡大学医学院药理学教研室(宜昌443oo2) 摘 要 目的：研究益舒软肝丸逆转肝硬化的作用机理。方法：以CCL4复合因素法 建立肝硬化模型，首先制备出肝硬化的实验动物模型，造模结束后，连续灌胃给药3个 月。检查ALT、AST，A／G、CHE；HA、LN、PllINP、Hyp；SOD、MDA。结果：与模型对照组大 鼠比，用药组组织切片显示汇管区有少量纤维组织增生，但未见假小叶结构；肝功能指标 ALT、AST减少，A／G、CHE增加，肝功能有显著好转；HA、LN、PllI NP、Hyp水平显著降低， 肝纤维化程度降低；MDA水平降低。结论：说明益舒软肝丸有明显的抗肝硬化的作用，作 用机制可能与抗脂质过氧化，保护肝细胞有关。 关键词 益舒软肝丸；肝硬化；逆转；中医药疗法；实验研究 Experimental Study on Yishuruanganwan’S Anti—lipoperOxidatiOn in Rats Chen Chao Wang Xijun Department of Pharmacology，Medical Science College of China Three Gorges University，Yichang 443002，China Abstract 0bjective：To investigate the effect and mechanism of Yishuruan— ganwan(YS)on liver fibrosis induced by CCL4．The main contents are as follows． Methods：80 healthy clean rats were divided into normal group，model group． The animal models were induced by complex factors of CCL4．foods of high fat．At the end of inducing model，model group divid into model group，three different dose groups．The course of treat is 1 2 weeks．Detect the degress of histopathology changes of liver in CCL4一induced cirrhosis．the indices of liver and spleen，and the level of ALT、AST and HA、LN、PIll NP in serum，and the level of A／G 、CHE， and the content of Hyp and MDA in liver tissue induced by CCL4．Results：Com． paring with the model group，the degress of histopathology changes of liver in CCL4一induced cirrhosis were ameliorated remarkably by YS treatment．the de— creased indices of liver and spleen。and the decreased level of ALT、AST and HA、 LN、P Ill NP in serum，and the increased level of A／G 、C