等渗胁迫下PEG和NaCl对野大豆萌发的影响.pdfVIP

安徽农业科学，Journal of Anhui Agri．Sci．2007，35(35)：11378，11408 责任编辑 金琼琼 责任校对 王 森 等渗胁迫下PEG和NaCI对野大豆萌发的影响 王丽燕 (德州学院生物系，山东德州253o23) 摘要 [目的】阐明干旱胁迫与盐胁迫的关系，为整体性抗逆研究提供参考。『方法】分别用NaC1、PEG模拟盐胁迫和干旱胁迫，测定等渗 透胁迫下野大豆种子的萌发率、发芽势、发芽指数和生长情况。『结果]PEG处理抑制了野大豆的萌发，计数第1天一0．4、一0．8 MPaPEG 处理的发芽率均明显低于对照。一0．4MPaNaC1促进野大豆的萌发，一1．6MPaNaC1处理发芽率明显降低。低浓度的PEG和NaC1对萌 发率影响不大，高浓度的PEG和NaC1降低发芽率，且等渗的PEG对野大豆的发芽率抑制强于NaC1。发芽势、发芽指数的变化与发芽 率的变化相似，PEG和NaI=l降低种子的萌发势。『结论1野大豆种子对盐胁迫具有更大的萌发耐受能力。在该试验浓度范围内，影响盐 生植物种子萌发的因素主要是渗透胁迫。 关键词 野大豆；PEG；NaC1；发芽率；发芽势；发芽指数 中图分类号 Q945．78 文献标识码 A 文章编号 O517—6611(2007)35—11378一O1 Effects ofPEG and NaCl on the Germination ofGlycine soja under Iso-osmotic Stress WANG Li-yan (Department of Biology，Dezhou University，Dezhou，Shandong 2530231 Abstract 『0biective1 The aim of the research was to clarify the relationship of drought stress and salt stress and provide basis for the research on stress resistance as a whole 『Method1 NaC1 and PEG were used to simulate salt stress and drought stress to determine germination rate，the germination potential，the germination index and growth conditions of G ine soja seeds under iso-osmotic stress．【Result】PEG treatment inhibited the germination of G．soia and the germination rate of seeds treated with一0．4、一0．8 MPa PEG in the 1st day of counting were obviously lower than that of CK．Treatment with一0．4 MPa NaCl promoted the germination of G．soja and the germination rate in the treatment with一1．6 MPa NaCl was obviously reduced．Low concentration of PEG and NaCl had little effects on the germination rate．high concentration of PEG and NaCl reduced the germination rate and the inhibition effect of iso-osmotic PEG on the germination rate of G．soja was stronger than that of NaC1． The changes of the germination potential and the germination index were similar to that of the germination rate and PEG and NaC1 reduced seed germination potentia1．『Conclusion1 The seeds of G．soja had g