缓冲QuEChERS法提取气相色谱火焰光度检测法快速测定水果蔬菜中39种有机磷农药残留.pdfVIP

中国卫生检验杂志2007年12月第l7卷第l2期 Chinese Journal of Health Laboratory Technology，Dec 2007；Vol 17 No 12 2133 【论著】 缓冲QuEChERS法提取气相色谱火焰光度检测法快速 测定水果蔬菜中39种有机磷农药残留 张秀尧，蔡欣欣，陈珞洛，郑三燕 (浙江省温州市疾病预防控制中心，浙江温州 325001) [摘要】 目的：建立快速测定水果蔬菜中常见的39种有机磷农药残留的气相色谱火焰光度检测方法。方法：10 g样品 用 10 ml 0．1％乙酸的乙腈快速提取、无水醋酸钠和无水硫酸镁盐析后，经PSA填料分散性固相萃取净化，提取液用等量 的乙酸乙酯稀释，气相色谱火焰光度法检测，基体标准定量。结果：39种有机磷农药的加标回收率在74．3％～114％，相 对标准偏差在3％～12％之间，检出限均小于0．01 mg／kg。结论：本法快速、简单、便宜、有效、稳健和环保，适合于水果蔬 菜中有机磷农药多残留分析。 [关键词】 QuEChERS；气相色谱火焰光度检测法；多残留分析；有机磷农药；水果；蔬菜 [中图分类号】 0657．7 1 [文献标识码】 A [文章编号】 1004～8685(2007)12—2133—03 GC——FPD determination of 39 organophosphoru pesticide residues in frui~ and vegeta·- bles using buffered QuEChERS Zhang Xiu—yao，Cai Xin·xin，Chen Luo·luo，Zheng San—yan (Wenzhou Center for Disease Control and Prevention，Wenzhou 325001，China) [Abstract] Objective：To develop a simple，fast and inexpensive GC—FPD method for the determination of39 organophospho- ru pesticide residues in fruits and vegetables．Methods：The procedure involved the extraction of 10 g sample with 10 ml acetoni— trile containing 1％ acetic acid．followed by liquid—liquid partitioning formed by adding 4 g anhydrous magnesium sulfate plus 1 g sodium acetate．Mter being processed by a simple cleanup step known as dipersive solid—phase extraction，the extract Was de— luted with EtOAc equally，then detected by GC—FPD，and quantified by matrix—match standard solution．Results：Recoveries between 74．3％ and 1 14％ and RSD ranged from 3％ to 12％ have been achieved．The limits of detection for all 39 organophos— phoru pesticides were within 0．01 mg／kg．Condusion：The method is quick，easy，cheap，effective，stable and safe， [Key words] QuEChERS；GC—FPD；Pesticide muhiresidues analy