五节芒定居对尾矿砂重金属形态、微生物群落功能及-植物生态学报.PDF

植物生态学报 2009, 33 (4) 629~637 Chinese Journal of Plant Ecology 五节芒定居对尾矿砂重金属形态、微生物 群落功能及多样性的影响 张崇邦 王 江 柯世省 金则新 (浙江省台州学院生命科学学院,浙江临海 317000) 摘 要 选择尾矿砂裸地和4个五节芒(Miscanthus floridulus)定居地(RI、RII 、RIII和RIV)为样地, 分别研究了五节 芒定居对尾矿砂重金属形态转化和微生物功能参数的影响。结果表明: 五节芒自然定居显著地提高了尾矿砂碳酸 盐结合态和硫化物-有机物结合态重金属比例(p 0.05), 降低了尾矿砂残渣态重金属的比例(p 0.05) 。土壤微生物群 落的纤维素分解作用、酚转化作用、固氮作用、氨化作用、硝化作用、有机磷转化作用、功能多样性、4类不同 碳源(碳水化合(CH)、聚合物(PL)、胺类化合物(AM)和杂合物(ML))均随着五节芒自然定居显著提高(p 0.05) 。而 氨基酸(AA)的利用强度却显著下降(p 0.05) 。典范相关分析(CCA)表明: 土壤微生物功能参数的总体变化与土壤碳 酸盐结合态和硫化物-有机物结合态重金属的含量呈显著正相关, 与残渣态重金属含量呈显著负相关。该研究结果 表明, 五节芒定居不仅促进了尾矿砂重金属朝着沉淀态和螯合态方面转化, 而且还显著地改善了尾矿砂微生物群 落的功能发挥。因而, 五节芒在重金属矿业废弃地恢复实践中具有较大的应用潜力。 关键词 五节芒 尾矿砂 重金属形态 微生物群落功能及其多样性 EFFECTS OF NATURAL INHABITATION BY MISCANTHUS FLORIDULUS ON HEAVY METAL SPECIATIONS AND FUNCTION AND DIVERSITY OF MI- CROBIAL COMMUNITY IN MINE TAILING SAND ZHANG Chong-Bang, WANG Jiang, KE Shi-Xing, and JIN Ze-Xin School of Life Sciences, Taizhou University, Linhai, Zhejiang 317000, China Abstract Aims Our objectives are to 1) evaluate the transformation of heavy metal speciations, the mineralization of soil microbial community and diversity of soil microbial community along an inhabi- tation gradient of Miscanthus floridulus and 2) test relationships between heavy metal speciations and microbial parameters during natural inhabitation by M. floridulus . This is the first step towards large scale application of M. floridulus to the revegetation of mine tailings. Methods On the basis of the cover of M. floridulus, four levels of inhabitation were selected, along with one bare site for comparison. Three sample plots w