武汉工程大学研究生试卷本.doc

武 汉 工 程 大 学 （硕士）研究生试卷本 考试课程名称＿＿＿＿＿＿＿＿＿＿ 考 试＿＿＿＿＿＿＿＿＿＿ 考 查＿＿＿＿＿＿＿＿＿＿ 学 科 专 业＿＿＿＿＿＿＿＿＿＿ 学 号＿＿＿＿＿＿＿＿＿＿ 姓 名＿＿＿＿＿＿＿＿＿＿ 题 题目序号 1 2 3 4 5 6 7 8 9 10 总 总计 评卷 评卷教师 各题分数 Preparation of Polysaccharide-coating Type Chiral Stationary Phases Since 1980s, chiral high-performance liquid chromatography(HPLC) for separating the enantiomers of racemates has attracted increasing attention (Petersen 2015, Lorenz, Seidel‐Morgenstern 2014, Martens, Bhushan 2014, Shen, Okamoto 2015, Kong, Zhang et al. 2015, Kuang, Ma et al. 2014, Zhang, Zhang et al. 2014). This technique is usually employed to determine enantiomer contents, or to prepare pure enantiomers (Gallinella, Ferretti et al. 2016, Woods, Patel et al. 2014, Zhang, Gil et al. 2015). Chiral stationary phases(CSPs) play an essential role for the enantioseparation by HPLC (Lorenz, Seidel‐Morgenstern 2014, Martens, Bhushan 2014). In this field, the major works are concerning the development of various types of CSPs, which are desired to separate the enantiomers of a wide range of chiral compounds (Guntari, Nam et al. 2014, Kreituss, Bode 2016, P?otka, Biziuk et al. 2014, Poiger, Mu?ller et al. 2015, Suliman, Elbashir et al. 2015, Suzuki, Han et al. 2015). Generally the well-known CSPs are prepared by coating polysaccharide derivatives on solid supports(mostly 3-aminopropyl silica gel), or by covalently immobilizing low-molecular-weight chiral molecules, synthetic chiral polymers, or polysaccharide derivatives on functionalized silica gel (Chen, Duan et al. 2014, Guesdon, Avrameas 2014, Junter, Thébault et al. 2016, Mohan, Rathner et al. 2015). From the point of practicable application view, the methods to prepare CSPs by coating polysaccharide derivatives on supports are more preferable, because polysaccharides, such as cellulose, amylose and chitosan, etc. are readily available (Lorenz, Seidel‐Morgenstern 2014, Kuang, Ma 2014, Chen, Duan 2014). Moreover, the CSPs can be easily prepared in