生物大分子结构与功能第2章相互作用力第3节第4节.ppt

1, re-visit of the secondary structures 2, Super secondary structures and domains 3, Tools to investigate the protein conformation 4, globular proteins and SCOP 5, fibrous proteins Helpful websites: 1, PDB (Protein Data Bank) /pdb/index.html 2, SCOP (Structural Classification of Proteins) http://scop.mrc-lmb.cam.ac.uk/scop/ 3, comparison of protein structures in 3D http://www.ebi.ac.uk/dali/index.html A, X-ray crystallography B, NMR (nuclear magnetic resonance) C, CD (circular dichroism) D, … Computer programs NMR and Nobel Price: 1944: I. I. Rabi, suggests that information about atoms nuclei can be obtained by studying the internal magnetism of protons. This forms the fundamental basis for todays resonance imaging technologies 1952: Physicists E. Purcell (Harvard) and F. Bloch (Stanford) discover Nuclear Magnetic Resonance (NMR).  1991: R. Ernst, Advances in NMR could lead to the ability to directly observe the chemical action of medication in the body. 2002: John B. Fenn, Koichi Tanaka, Kurt Wüthrich for the development of nuclear magnetic resonance spectroscopy for determining the three-dimensional structure of biological macromolecules in solution ????????????????????? The Nobel Prize in Chemistry 2002 for the development of methods for identification and structure analyses of biological macromolecules for their development of soft desorption ionisation methods for mass spectrometric analyses of biological macromolecules for his development of nuclear magnetic resonance spectroscopy for determining the three-dimensional structure of biological macromolecules in solution ??????????????????????????????? ??????????????????????????????? ??????????????????????????????? John B. Fenn Koichi Tanaka Kurt Wüthrich ??? 1/4 of the prize ??? 1/4 of the prize ??? 1/2 of the prize USA Japan Switzerland Virginia Commonwealth University Richmond, VA, USA Shimadzu Corp. Kyoto, Japan Eidgen?ssische Technische Hochschule (Swiss Federal Institu