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病毒学实验技术Techniques in Virology
Techniques in Virology;viruses are usuallydetected by indirect methods;Sampling
Injection in animal models (diseases), or eggs
Virus culture in mosquito or mammalian cells
Detection of virus replication
Cytopathic effect (syncitia, inclusion bodies, cell rounding, apoptosis, microarrays)
Hemadsorption
Antigen detection (immunocapture, Dot-blot, Western blot, Immunofluorescence,
Immunohistochemistry, ELISA
Electron microscopy
Genome detection (Electrophoresis, in situ or membrane Hybridization, RT-PCR, `
multiplex or real time PCR, NASBA, Hybrid RNA-DNA, microarrays, Sequencing,
substraction sequencing, random amplification-cloning-high throughput
sequencing-blast)
Reporter gene in genetically engineered cell lines;Specimens:
Blood: serum, plasma, PBMC
Swabs: Tracheal, nasopharyngeal, cutaneous…
Fluids: CSF, urine, bronchoalveolar lavage, tracheal aspirate, tears…
Tissues: Organs, skin… ;Sampling
Injection in animal models (diseases), or eggs
Virus culture in mosquito or mammalian cells
Detection of virus replication
Cytopathic effect (syncitia, inclusion bodies, cell rounding, apoptosis, microarrays)
Hemadsorption
Antigen detection (immunocapture, Dot-blot, Western blot, Immunofluorescence,
Immunohistochemistry, ELISA
Electron microscopy
Genome detection (Electrophoresis, in situ or membrane Hybridization, RT-PCR, `
multiplex or real time PCR, NASBA, Hybrid RNA-DNA, microarrays, Sequencing,
substraction sequencing, random amplification-cloning-high throughput
sequencing-blast)
Reporter gene in genetically engineered cell lines;Choosing a culture system for animal viruses; 病毒的培养;tracheal organ cultures;Growing viruses in the lab;Cell culture;Animal virus plaques in a cell culture;Phage plaques in a lawn of bacterial cells;Plaques formed by a phage in a bacterial lawn.
The control plate on the left was inoculated with only the bacterial host.
The plate on the right was inoculated with phage and bacterial host. ;14;Plaques formed by West Nile v
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