研究生-2014现代分子生物学英语.ppt

Conclusions Agrobacterium is an amazing organism, with a unique ability to transfer DNA into diverse host genomes, which has been exploited to facilitate research and breeding Agrobacterium research and its application went far beyond what Smith and Townsend could foresee when they found crown gall was caused by the bacterium in 1907 Edward L. Barnard, Florida Department of Agriculture and Consumer Services, B; Mike Ellis, Ohio State University; Agrobacterium-mediated transformation is an important tool for research Casimiro, I., Marchant, A., Bhalerao, R.P., Beeckman, T., Dhooge, S., Swarup, R., Graham, N., Inzé, D., Sandberg, G., Casero, P.J. and Bennett, M. (2001). Auxin transport promotes Arabidopsis lateral root initiation. Plant Cell. 13: 843-852. Genome-Wide Insertional Mutagenesis of Arabidopsis thaliana (Published 2003; cited 2000 times) Gene T-DNA inserts Arabidopsis transformed with an auxin-inducible reporter gene Agrobacterium can accelerate crop breeding Poor tomato but disease resistant Elite tomato Elite, disease resistant tomato Agrobacterium-mediated transformation allows a single gene to be introduced into a genome. This method can be faster than conventional breeding transformation Agrobacterium enables novel or modified genes to be introduced Golden rice is a nutritionally enhanced plant Transgenic plant expressing insecticidal Bt gene Wild-type peanut plant Peanut plant expressing the Bt gene Bacillus thuringiensis expressing Bt toxin Plant cell expressing Bt toxin Photo credits: Herb Pilcher, G Future directions Nester, E., Wood, D., and Liu, P. 2004. Agrobacterium-plant interactions: unfinished business and continuing surprises. Proceedings of the 11th International Congress on Molecular Plant-Microbe Interactions, St. Petersburg, Russia, July 18 - 26, 2003. Biology of Plant-Microbe Interactions, Vol 4; Edward L. Barnard, Florida Department of Agriculture and Consumer Services, B; . Ongoing research includes The process for T-DNA integr