包埋-脱水法常温和常低温保存条斑紫菜自由丝状体.pdfVIP

安徽农学通报，Anhui A ．Sci．Bul1．2007，13(23) 包埋一脱水法常温和常低温保存条斑紫菜自由丝状体 王 欢 曹光辉 高艳萍 王起华 (辽宁师范大学生命科学学院，辽宁大连 1 16029) 摘 要：用包埋一脱水法在常温和常低温下保存条斑紫菜自由丝状体，探讨了温度、光(暗)和含水量等因素对存活率 的影响。结果表明，条斑紫菜自由丝状体在常温或常低温下都可以保存6个月并保持较高的存活率；其中，4E(暗) 保存的最高存活率达到71，5％；而且保存后的丝状体的生长力达到了保存前的水平。包埋一脱水法操作简单，无需 贵重设备，在藻类种质保存中有广阔的应用前景。 关键词：条斑紫菜，自由丝状体，包埋一脱水法，常低温保存 中图分类号 文献标识码 B 文章编号 1007—7731(2007)23—86一o2 Storage of the Free Living Conchocelis of Porphya Yezoens~Ueda by Encapsulation——Dehydration under Normal and Norm al Low Temperature Wang Huan Cao Guanghui Gao Yanping Wang Qihua (School of Life Sciences，Liaoning Normal University，Dalian， Liaoningl 16029，China) Abstract：The free living conchocelis of Porphy~yezoensis Ueda were preserved in normal temperature and normal low tem- perature by using encapsulation—dehydration method．The facters influencing the algal viability．such as different tempera- ture．illumination intensities and the water content of beads were studied．The results showed that the conchocelis were stored f0r 6 months under normal temperature and normal low temperature and the high viability were obtained．．the algae viability reached the most point at 71．5％ in the temperature of 4℃(dark)，furthermore，the algae viablity of the conchocelis pre- served could reach the normal leve1．The method of encapsulation—dehydration simplifies the operation procedure and do not need expensive equipments，SO it is a potential method for preservation of the algae． Key words：P0，p^，．r yezoensis，free living conchocelis，encapsulation—dehydration，normal low temperature preservation 紫菜不仅营养丰富，具有多种药用和保健功能，还可 学重点实验室提供。无菌条件下培养在装有150mL PES 以作为工业原料，具有重要的经济价值…。紫菜在我国的 培养基的250mL三角瓶中。培养条件为20±2qC，光强 海藻养殖中占有重要地位 。由