北京市大气PM10和PM2．5对人肺成纤维细胞间隙连接通讯及连接蛋白的影响.pdfVIP

· 584 · 环境与职业医学2007年l2月第24卷第6期 J Environ Oecup Med，Dec．2007 Vo1．24 No．6 文章编号：1006—3617(2007)06—0584—05 中图分类号：R122 文献标识码：A 【论著】 北京市大气PM。0和PM2．5对人肺成纤维细胞 问隙连接通讯及连接蛋白的影响 赵晓红 ，贾玉巧2 7郭新彪 摘要： [目的]探讨北京市大气颗粒物PM．。和PM 对人肺成纤维细胞(HLF)间隙连接通讯(GJIC)及间隙连接蛋 白(Cx43)的影响。 [方法]颗粒物样品采自北京市城区采暖期，实验细胞为HLF。采用划痕染料标记示踪法(SLDT)测 定HLF的G．IIc的水平；蛋白免疫印迹(westen bloting)法观察HLF细胞膜上Cx43的表达。 [结果]细胞划痕实验结果显 示，PM。。和PM 均可抑制细胞问荧光扩散，抑制作用随剂量增高而增强；蛋白免疫印迹结果显示，PM．。和PM： 可使细胞 膜Cx43表达减少，减少量随浓度的增高而增加。 [结论]北京市大气颗粒物PM．。和PM 能抑制HLF的GJIc，抑制的机 制可能与Cx43表达抑制有关。 关键词：PMl0；PM2 ；人肺成纤维细胞(HLF)；细胞间隙连接通讯(GJIC)；间隙连接蛋白(Cx43) Effects ofPMlo andPMzs on Gap JunctionalIntercellular Communication and Cx43 in HLF cell ZHAo Xiao—hong ．． Yu—qiao ．GUO 一biao (1．Beoing Key Laboratory ofBioactive Substances and Functional Foods College ofAppliedArts andScience，Be~iing Union University，Beijing 100083，China；2．Department of Toxicology，Baotou Medical College，Baotou 014010，China；3．Department of Occupational and Environmental Health Sciences，School ofPublic Health，Peking University，Beijing 100083，C ina) Abstract： [Objective j To investigate the effect of PMl0 and PM2 5 on Gap Junctional Intercellular Communication (GJIC)and the expression of the gap iunction protein Cx43 in Human Lung Fibroblasts(HLF)．[Methods]PM10 and PM2 5 were collected from Beijing urban traffic road in heating season．The inhibition effect of PMl0 and PM2 5 on GJIC was studied by the scrape loading dye transfer assay，and the expression of Cx43 in HLF was analyzed with Western Blotting assay．[Results] ( IC between human lung fibroblasts was significantly inhibited by PMl0 and PM2 5 in different concentrations． rhe inhibitory effect of PM2 , 5 on GJIC was stronger than PM【0．PM10 and PM2 5 COuld decrease the expression of th