十二烷基聚氧乙烯醚降解菌的分离、鉴定及降解特性.pdfVIP

中国环境科学 2007，27(6)：768-772 China Environmental Science 十二烷基聚氧乙烯醚降解菌的分离、鉴定及降解特性 杨海君’，杨成建 ，肖启明’ (1．湖南农业大学生物安全科技学院，湖南长沙410128；2．重庆文理学院化学与环 境科学系，重庆 402 1 68) 摘要：从工厂排污口废水中分离 纯化并筛选出一株降解十二烷基聚氧乙烯醚(B嘀一3o)的菌株，鉴定为伯克氏菌属(Pandoraea )，命名为 B30．当温度为3O℃，pH5～8，底物浓度低于0．2g：L，且以蛋白胨做氮源时，菌株B30降解效果最好；Zn”、ca2 、AI 、Fe 对菌株B30的生长 及其降解性能具有较大的促进作用．在低浓度区(O．05--0．20g／L)，菌株B30生长最快，降解活力性最强，12h内能将底物基本降解；在中等浓度区 (O．40g／L左右)，72h内能降解50％的底物；在高浓度区(0．8 1．50#L)，72h内底物的降解率在30％以下． 关键词：筛选；16S rDNA；降解特性；十二烷基聚氧乙烯醚 中图分类号：X51 文献标识码：A 文章编号：1000—6923(2007)06—0768 05 Isolation,identification of a alkyl-polyoxyethylene degrading bacterium strain and its degradation characteristics． YANG Hai~un ，YANG Cheng-jian2，XIAO Qi—ming (1．College of Bio-safety Science and Technology,Hunan Agricultural University,Changsha 410128，China；2．Department of Chemistry and Environment Science，Chongqing University ofArts and Sciences，Chongqing 402168，China)．China Environmental Science,2007，27(6)：768～772 Abstract：A bacterial strain B30 of degrading 12 alkyl-polyoxyethylene(Brij-30)was isolated，purified and sorted from the wastewater in pollution-outlets of a factory，and identified as Pandoraea sp．．At temperature of 30℃，pH of 5-8， B哟一30 concentration lowered 0．20g／L，protein peptone as the nitrogen source，bacterial strain B30 degradation effect Was the best．Zn2 、C 、AI 、Fe had greater promoting effect on the growth of bacterial strain B30 and its degradation property．At low concentration area(0．05--0．20g／L)，bacterial strain B30 grew the quickest，degradation activity Was the strongest，could degrade basically Brij一30 in 1 2h；in medium concentration area(about 0．40g／L)，could degradate 50~,4 Brij-30 in 72h；in high concentration area(0．80~1．50g／L)，the degradation rate in 72h Was lower under 30％． Key words：screening；16S rDNA~degrading characteristics：alkyl-polyoxyethylene 非离子型表面活性剂是一类聚氧乙烯型或 供资料． 多元醇型表面活性剂，其在自然环境中的归宿、 1 材料与