南极海单胞菌BSw10005代谢活性物质及对植物病原菌抑制作用.pdfVIP

植物病理学报 ACTA PHYTOPATHOLOGICA SINICA 37(6)：654-659(2007) 南极海单胞菌BSw1 0005代谢活性物质 及对植物病原菌抑制作用 任雪芸 ，王 伟 ，郭少华 ，李元广 ，陈 波 ( 华东理工大学海洋生化32程研究所生物反应器工程国家重点实验室，上海 2~237； ( 中国极地研究中心，上海200129) 摘要：对从南极海水中分离的海单胞菌BSwl0005发酵液进行冷冻干燥、乙醚萃取，获得白色固体活性粗提物，其对l5种 植物病原真菌、细菌和海洋病原弧菌等都具有较好的抑制作用。对西瓜枯萎病菌(Fusarium D D )的抑制表现为使菌 丝中原生质凝聚、菌丝体壁破裂和抑制分生孢子萌发。该活性物质在30～100oC范围内稳定性较好，在pH值2～8的条件 下具有较好的活性，说明该活性物质具有作为农用抗生素的潜力。该抑菌物质经过官能团显色反应、凝胶柱层析、薄层层 析、红外光谱和元素分析，初步判定为胺类物质。 关键词：南极海单胞菌；活性物质；植物病原菌；抑菌作用 Bioactive substance of Marinomonas sp。BSwl 0005 frOm Antarctic ocean and its effects on plant pathogens REN Xue—yun ，WANG Wei ，GUO Shao—hua ，LI Yuan—guang ， CHEN Bo ( Institute of Marine Bioprocess Engineering，East China University of Science and Technology／State Key Labo— ratory of Bioreactor Engineering，Shanghai 200237，China； Polar Research Institute of China，Shanghai 200129，China) Abstract： A white bioactive substance was isolated from liquid culture of Marinomonas sp．BSwl0005 isola— ted from Antarctic ocean．The antibiotic substances showed strong anti—microbal activities against 15 plant pathogens and marine pathogenic Vibrio spp．．The effect of the antibiotic substances on Fusarium o~ omm was investigated．The results showed that the mycelial growth and conidial germination of F．o~ omm were significantly inhibited after treatment with the antibiotic substances．The treated hyphal cells of F．o~ omm became broken and protoplasm agglomerated．Th e anti—microbal components remained active in acid solutions from pH 2 to 8 and temperature from 30oC to 100℃．These results suggested that Marinomonas sp．BSwl0005 had the potential as a bio—control agent．Further analysis by color reactions，SEC，IR spectrum and element analysis indicated that the antibiotic substances were amines． Key words： antarcitic Marinomonas sp．；bioactive substance；plant pathogen；antifungal action 中图分类号：Q178．53；S476