《天然产物的分离与纯化》结课论文.docx

摘要本文是以采用乙醇/硫酸铵双水相体系萃取荞麦茶中总黄酮，对不同条件下的萃取情况进行研究，最终建立乙醇/硫酸铵双水相体系萃取分离的最佳工艺条件。其主要的实验原理在于以荞麦茶为原料，用芦丁为对照品，采用乙醇/硫酸铵双水相体系萃取荞麦茶中总黄酮。考察乙醇浓度、硫酸铵浓度、料液比等对总黄酮萃取效果的影响。关键词：总黄酮 荞麦茶 乙醇/硫酸铵双水相体系萃取法 条件工艺改进In this paper, the total flavonoids in buckwheat tea were extracted by ethanol / ammonium sulfate aqueous two-phase system, and the extraction conditions under different conditions were studied. Finally, the optimum extraction conditions of ethanol / ammonium sulfate aqueous two-phase system were established. The main experimental principle is to buckwheat tea as raw material, with rutin as the reference substance, using ethanol / ammonium sulfate two-phase system extraction of total flavonoids in buckwheat tea. The effects of ethanol concentration, ammonium sulfate concentration, ratio of material to liquid on the extraction of total flavonoids were investigated.Key?words:?total?flavonoids?buckwheat?tea?ethanol?/?ammonium?sulfate?aqueous?two-phase?system?extraction?process conditions process?improve前言荞麦茶是以荞麦为原料，经杀青、揉捻、干燥等工艺加工而成 [1] 。荞麦茶中含有丰富的蛋白质、矿物质和黄酮等生物活性物质。黄酮具有抗氧化、改善血液循环、降低胆固醇、改善心脑血管疾病症状、杀菌消炎等功效 [2] 。因此，开展对黄酮类化合物的提取工艺的研究具有十分重要的现实意义。超声提取技术主要是利用超声波的空化作用加速有效成分的浸出提取，同时超声波的次级效应也有利于提取。超声波提取总黄酮具有省时、节能、提取率高等优点。本文采用超声波溶剂法提取总黄酮，以总黄酮含量为考察指标，确定了较优的工艺条件。双水相萃取依据组分在两相间的选择性分配实现目标成分的分离，因其萃取分离过程条件温和，可调节因素多，易于放大和操作，特别适用于生物物质的分离和提纯 [3-4] 。已有研究表明：异丙醇、乙醇等低成本的普通有机物与无机盐和水以适当浓度混合时，也能形成双水相体系 [5-6] ，本文研究了茶多酚在乙醇/硫酸铵双水相体系的上下相中的不同分配情况，茶多酚在上相中富集，这样由于茶多酚上下相中含量的差异，可以获得较高茶多酚萃取率的最佳双水相萃取的工艺条件。该试验对于茶多酚的富集和提取有很大的意义，对于茶多酚的大规模生产和广泛应用具有很重要的实际意义。目录概论................................................................................................................4黄酮提取分离与纯化方法的研究................................................................5实验部分........................................................................................................8结论与展望...................................................................................................10致谢..........................................................................