减量训练对大鼠骨骼肌PI3KAkt表达的影响.pdfVIP

(4)Compared with group C4, The protein expression of PI3K p85 of gastrocnemius muscle in group E4 highly significantly increased(p0.01)and the protein expression of PI3K p110αsignificantly increased(p0.05).Akt protein and phos-GSK3β(Ser9) trended to increase. Conclusions: (1)Heavy load training inhibit rat’s body weight and the PI3K/Akt/ GSK3β pathway of skeletal muscle; (2)The protein expression of PI3K p110α、PI3K p85 and Akt of skeletal muscle was effectively elevated by reducing training, and the muscle protein synthesis was improved. (3)The overcompensation period of reducing training may be in three to six days after the training. Key words: reducing training;Akt;PI3K; GSK3β 论文随机验证编号：BT5431225377 VI 万方数据 目录 1 前言 - 3 - 1.1 选题依据 - 3 - 1.2 研究内容 - 3 - 1.3 研究技术路线 - 4 - 1.4 预期结果 - 4 - 2 文献综述 5 2.1 PI3K 5 2.1.1 概述 - 5 - 2.1.2 PI3K 对骨骼肌蛋白代谢的影响 - 6 - 2.2 Akt 7 2.2.1 概述 7 2.2.2 Akt 对骨骼肌蛋白代谢的影响 8 2.3 GSK3β 9 2.3.1 概述 9 2.3.2 GSK3β 对骨骼肌蛋白代谢的影响 10 2.4 运动对 PI3K/Akt 信号通路的影响 10 2.4.1 不同运动对 PI3K/Akt 信号通路的影响 10 2.4.2 减量训练对 PI3K/Akt 信号通路的影响 12 3 研究方法 13 3.1 研究对象、分组及训练方案 13 3.1.1 研究对象及分组 13 3.1.2 训练方案 13 3.2 取材和前处理 15 3.3 测试指标及测试方法 15 3.3.1 肌肉匀浆制备 15