参附强心合剂对心衰大鼠肾素、AngⅡ、醛固酮、NE及心肌组织病理变化调控作用的研究.pdfVIP

中文摘要 目的观察参附强心合剂对心衰大鼠神经内分泌系统、心肌组织病理学的影 响。 方法 选用SD 雄性大鼠72 只，采用腹主动脉缩窄法制造心衰模型，造模成 功的大鼠随机分为模型组、参附强心合剂大剂量组、中剂量组、小剂量组、西药 组、假手术组，灌胃8 周后，用放免法检测血浆和心肌肾素（PRA）、血管紧张素 II(Ang II)、醛固酮（ALD）含量，Elisa 法检测血浆和心肌的去甲肾上腺素(NE) 含量，并观察心肌组织的病理改变。 结果 病理切片见，参附强心合剂大剂量组、西药组心肌细胞坏死及凋亡均 显著下降；中药大剂量组及西药组与模型组相比能显著降低血浆和心肌PRA、Ang Ⅱ、ALD、NE 水平，有显著统计学差异（P 0.05）。 结论 参附强心合剂可有效抑制心肌细胞坏死及凋亡，降低PRA、Ang Ⅱ、ALD、 NE 的水平，从而对缺血心肌有保护作用，并呈一定的量效关系。 主题词 心力衰竭，大鼠模型，参附强心合剂， PRA，ang-II，ALD，NE I 万方数据 ABSTRACT Objective To investigate the effect of shenfuqiangxin decoction on Neuro-endocrine activation and histological in experimental rats with heart failure(HF). Methods Rat models of heart failure were established by coarctating abdomial aorta .After that, 60 male SD rats were divided into six group randomly: the model group，the low – dose shenfuqiangxin Decoction group, high - dose shenfuqiangxin Decoction ,the control group and the western medicine group. Then rats were treated for 8 weeks. the changes of heart morphological structure, the plasma and myocardial level of Renin 、angitensin II、Aldosterone 、notepinephrin were observed and measured. Results After treatment of shenfuqiangxin Decoction , the plasma and myocardial level of Renin、angitensin II、Aldosterone 、notepinephrin were decreased more significantly than the model (P0 ．05).As compared with control group ,there were lower necrotic myocardial cells and cell death by apoptosis in high - dose shenfuqiangxin Decoction and western