冷暴露对中缅树鼩适应性产热特征的影响.PDF

兽类学报,2009,29 (1):50 - 58 Acta Theriologica Sinica 冷暴露对中缅树鼩适应性产热特征的影响 谢静 朱莉萍 王政昆 (云南师范大学生命科学学院,生物能源持续开发利用教育部工程中心,昆明 650092 ) 摘要:在冷暴露 (5 ± 1℃ ) 1 d,7 d,14 d 和21d 对中缅树鼩的肝脏、心脏、膈肌、腓肠肌和褐色脂肪组织 (BAT) 的总蛋白和线粒体蛋白含量、线粒体的状态Ⅳ呼吸能力、细胞色素C 氧化酶活力及血清中甲状腺激素的 水平等指标进行了测定。结果表明:冷暴露过程中肝脏线粒体蛋白含量对低温的反应比总蛋白的反应剧烈,心 脏和 BAT 组织的线粒体蛋白含量也随着冷驯化时间的延长而显著增加,但腓肠肌的反应较为温和;各组织线粒 体的状态Ⅳ呼吸能力均显著增强,除腓肠肌外其它各组织细胞色素C 氧化酶活性也随冷暴露时间的延长而急剧 增加。说明在低温条件下,肝脏、腓肠肌、心脏和 BAT 等组织都参与了体温调节的过程,在自然生境中,低温 是重要的刺激产热的调节因子。 关键词:中缅树鼩;适应性产热;冷暴露 中图分类号:Q494 文献标识码:A 文章编号:1000 - 1050 (2009 )01 - 0050 - 09 Effects of long-term cold-exposure on adaptive thermogenesis in the tree shrew (Tupaia belangeri)  XIE Jing,ZHU Liping,WANG Zhengkun (School of Life Science of Yunnan Normal University,Engineering Research Center of Sustainable Development and Utilization of Biomass Ener- gy,Ministry of Education ,Kunming 650092,China) Abstract :The total protein contents,mitochondrial protein contents,state Ⅳ respiratory ability,respiration control ratio (RCR ),Cytochrome C oxidase activity of liver,heart,musculus diaphragma gastrocnemius and brown adipose tissue (BAT)in tree shrews (Tupaia belangeri)and serum thyroid hormone levels were measured during cold exposure (5 ± 1℃ )for 1 day,7 days,14 days and 21 days. The results showed that the change of liver mitochondrial protein content was more acute than that for total protein. The mitochondrial protein content of heart and BAT were significantly increasedduring cold-exposure,however,the reaction of gastrocnemius was more moderate. The state IV mitochondrial respiration of these tissues increased more than did those of the control. The cytochrome C oxidase activity with cold acclimation also s