膨胀床离子交换吸附分离纳豆激酶.PDFVIP

33 4 Vol. 33 No. 4 2005 8 CHEM ICA L ENGINEERING ( CH INA) Aug. 2005 1 1 2 2 2 胡洪波, 张雪洪, 梅乐和 , 姚善泾, 朱自强 ( 1. , 200030; 2. , 310027) : , pH 6. 0, 6. 2 mS/ cm, 2 , ; , ; , , 6 2, 8) 10 h, 50% : ; ; ; : TQ 028. 8; TQ 033 : A : 1005-9954( 2005) 04-0001-04 Purification of nattokinase by ion exchange adsorption in expanded bed 1 1 2 2 2 HU H ong-bo , ZHANG Xue-hong , M E Le-he , YAO Shan-jing , ZHU Zi-qiang ( 1. College of L ife Science nd B iotechnology, Sh ngh i Ji otong Un iversity, Sh ngh i 200030, Ch in ; 2. Dep rtment of Chem ic l nd Biochem ic l Engineering, Zheji ng Un iversity, H ngzhou 310027, Zheji ng Province, Ch in ) Abstract: The use of n exp nded bed for purific tion of the n ttok in se from cel-l cont ined ferm ent tion broth w s investig ted. M ethod-scouting expermi ents, c rried out in p cked beds, show th t the optmi l pH for dsorption is 6. 0 nd conductivity of the feed-stock sh ll be low er th n 6. 2 m S/cm. In dsorption st ge of exp nded bed, wt o modes of feed-stock pp lic tion, performed w ith const nt bed exp nsion nd const nt flow-r te, w ere stud ied. The resu lt show s th t m int ining const nt degree of exp nsion during the dsorption st ge is more su it b le for n ttok in se purific tion bec use the viscosity of the feed-stock is smi il r to th t of equilibr tion buffer solution. In w sh ing st ge, the p rticul te re